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Sample GSM1019504 Query DataSets for GSM1019504
Status Public on Dec 31, 2016
Title HumanTumor_FreshFrozen_PRI_DH032
Sample type RNA
 
Source name human tumor, colorectal primary, fresh frozen tissue
Organism Homo sapiens
Characteristics primary tumor site: colorectal
metastatic tumor site: NA
molecular subgroup: MSCC 4
Extracted molecule total RNA
Extraction protocol Human tumors were flash frozen in OCT at time of surgical resection. An initial section was stained with Hematoxylin and Eosin for histological characterization of the tissue, and the sample was subsequently macrodissected to ensure > 80% tumor. Approximately 100 ug of tissue was macrodissected, and total RNA was isolated from the homogenized tissue using the RNAase Isolation Kit (Qiagen, Valencia, Ca).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The resulting biotin-labeled RNA samples were then hybridized with Affymetrix U133A 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using the Gene Array Scanner (Affymetrix) following the manufacturer’s instructions (Affymetrix, Santa Clara, CA).
Data processing The data were analyzed using the R statistical platform with the Bioconductor affymetrix package [R version 2.13.1 (2011-07-08) and Bioconductor version 2.9].
 
Submission date Oct 12, 2012
Last update date Dec 31, 2016
Contact name David Hsu
Organization name Duke University
Department Medical Oncology
Street address 905 S. LaSalle St. Rm 3008
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
 
Platform ID GPL570
Series (2)
GSE41568 A Molecular Profile of Colorectal Cancer to Guide Therapy [PDCCEs]
GSE51244 A Molecular Profile of Colorectal Cancer to Guide Therapy

Data table header descriptions
ID_REF
VALUE Log2 GC-RMA signal

Data table
ID_REF VALUE
1007_s_at 12.4776159
1053_at 9.602247883
117_at 7.761854424
121_at 9.550107404
1255_g_at 4.280206288
1294_at 8.840050478
1316_at 6.7474139
1320_at 6.400490882
1405_i_at 6.779428268
1431_at 5.311592516
1438_at 10.01033515
1487_at 10.38851439
1494_f_at 7.167254514
1552256_a_at 11.67093236
1552257_a_at 10.09440069
1552258_at 6.9617326
1552261_at 6.411633749
1552263_at 8.039362194
1552264_a_at 8.860842938
1552266_at 6.173574772

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1019504_PRI_DH032.CEL.gz 5.4 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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