|
| Status |
Public on Feb 01, 2013 |
| Title |
RNA-Seq(d0) |
| Sample type |
SRA |
| |
|
| Source name |
3T3-L1 preadipocytes
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed C57/BL6-129
cell type: 3T3-L1 preadipocytes
|
| Treatment protocol |
adipogenesis inducing cocktails
|
| Growth protocol |
3T3-L1 preadipocytes were routinely cultured in DMEM plus 10% BS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol and the sequencing libraries were constructed with ChIP-Seq DNA Sample Prep kit (Illumina) and were sequenced on Illumina HiSeq 2000 system.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
sample8
|
| Data processing |
Base-calling and alignment performed by Illumina Analysis Pipeline
Conversion to BED format by bed tools v2.16
Genome_build: NCBI37 (mm9)
Supplementary_files_format_and_content: bed format containing the genomic coordinates of each mapped read
|
| |
|
| Submission date |
Oct 10, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Kai Ge |
| E-mail(s) |
kai.ge@nih.gov
|
| Phone |
301-451-1998
|
| Organization name |
NIH
|
| Department |
NIDDK
|
| Street address |
10 Center Dr Rm 8N307
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE41455 |
Histone H3K9 Methyltransferase G9a Represses PPARĪ³ Expression and Adipogenesis [ChIP-Seq and RNA-Seq data] |
| GSE41457 |
Histone H3K9 Methyltransferase G9a Represses PPARĪ³ Expression and Adipogenesis |
|
| Relations |
| SRA |
SRX193359 |
| BioSample |
SAMN01760776 |
