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Sample GSM1017637 Query DataSets for GSM1017637
Status Public on Feb 01, 2013
Title RNA-Seq(d0)
Sample type SRA
 
Source name 3T3-L1 preadipocytes
Organism Mus musculus
Characteristics strain: mixed C57/BL6-129
cell type: 3T3-L1 preadipocytes
Treatment protocol adipogenesis inducing cocktails
Growth protocol 3T3-L1 preadipocytes were routinely cultured in DMEM plus 10% BS.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol and the sequencing libraries were constructed with ChIP-Seq DNA Sample Prep kit (Illumina) and were sequenced on Illumina HiSeq 2000 system.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description sample8
Data processing Base-calling and alignment performed by Illumina Analysis Pipeline
Conversion to BED format by bed tools v2.16
Genome_build: NCBI37 (mm9)
Supplementary_files_format_and_content: bed format containing the genomic coordinates of each mapped read
 
Submission date Oct 10, 2012
Last update date May 15, 2019
Contact name Kai Ge
E-mail(s) kai.ge@nih.gov
Phone 301-451-1998
Organization name NIH
Department NIDDK
Street address 10 Center Dr Rm 8N307
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL13112
Series (2)
GSE41455 Histone H3K9 Methyltransferase G9a Represses PPAR╬│ Expression and Adipogenesis [ChIP-Seq and RNA-Seq data]
GSE41457 Histone H3K9 Methyltransferase G9a Represses PPAR╬│ Expression and Adipogenesis
Relations
SRA SRX193359
BioSample SAMN01760776

Supplementary file Size Download File type/resource
GSM1017637_RNA_3T3-L1_0h.bed.gz 423.4 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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