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Sample GSM1017635 Query DataSets for GSM1017635
Status Public on Feb 01, 2013
Title Input for H3K9me2
Sample type SRA
Source name 3T3-L1 preadipocytes
Organism Mus musculus
Characteristics strain: mixed C57/BL6-129
cell type: 3T3-L1 preadipocytes
antibody: none
Treatment protocol adipogenesis inducing cocktails
Growth protocol 3T3-L1 preadipocytes were routinely cultured in DMEM plus 10% BS.
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. For ChIP antibodies, Anti-H3K9me2 antibody from Millipore (17-648) was used for ChIP and ChIP-Seq; for H3K27me3, Millipore (07-499); for H3K4me3, Millipore 07-473. ChIP was performed by following a protocol from R. Myers’ lab ( The sequencing libraries were constructed with ChIP-Seq DNA Sample Prep kit (Illumina) and were sequenced on Illumina HiSeq 2000 system. The ChIP-enriched regions were identified using SICER (Zang et al, 2009), an algorithm designed to capture domains of diffuse signals.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
Description sample6
Data processing Base-calling and alignment performed by Illumina Analysis Pipeline
Conversion to BED format by bed tools v2.16
Genome_build: NCBI37 (mm9)
Supplementary_files_format_and_content: bed format containing the genomic coordinates of each mapped read
Submission date Oct 10, 2012
Last update date May 15, 2019
Contact name Kai Ge
Phone 301-451-1998
Organization name NIH
Department NIDDK
Street address 10 Center Dr Rm 8N307
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platform ID GPL13112
Series (2)
GSE41455 Histone H3K9 Methyltransferase G9a Represses PPARγ Expression and Adipogenesis [ChIP-Seq and RNA-Seq data]
GSE41457 Histone H3K9 Methyltransferase G9a Represses PPARγ Expression and Adipogenesis
SRA SRX193357
BioSample SAMN01760774

Supplementary file Size Download File type/resource
GSM1017635_3T3L1_d0_input.bed.gz 2.0 Gb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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