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Sample GSM1017584 Query DataSets for GSM1017584
Status Public on Aug 31, 2014
Title Liver, rep3
Sample type RNA
Source name Liver
Organism Mus musculus
Characteristics strain background: C57BL/6
tissue: Liver
replicate: 3
Treatment protocol B cells were purified from spleen using CD19-microbeads (MB) (Miltenyi Biotec). Liver was dissected and washed in PBS then was snap frozen on dry ice.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using the Rneasy kit (Qiagen).
Label cy3
Label protocol Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to Agilent’s protocol.
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slides were washed and then scanned with an Agilent Scanner.
Description Sample5
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters (protocol GE1-v5_95_Feb07 and Grid: 014868_D_F_20060807) to obtain background subtracted and spatially detrended Processed Signal intensities.
Submission date Oct 10, 2012
Last update date Aug 31, 2014
Contact name Deepak Mav
Organization name Sciome LLC
Street address 2 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
Platform ID GPL7202
Series (2)
GSE41449 Epigenetic regulation of gene expression is instructed by promoter sequence and refined by developmental progression [expression]
GSE41450 Epigenetic regulation of gene expression is instructed by promoter sequence and refined by developmental progression.

Data table header descriptions
VALUE The gProcessedSignal was log2-transformed and qunatile normalized to correct for between sample variation.

Data table
A_51_P100021 1.997417048
A_51_P100034 11.88508701
A_51_P100052 2.732961002
A_51_P100063 3.963825124
A_51_P100084 5.779738788
A_51_P100099 6.290430774
A_51_P100155 10.44303779
A_51_P100174 5.472195357
A_51_P100181 5.244753775
A_51_P100218 1.674996987
A_51_P100227 7.643456305
A_51_P100238 1.783901864
A_51_P100246 10.21342141
A_51_P100289 8.21708049
A_51_P100298 2.929515259
A_51_P100309 1.963370768
A_51_P100327 8.077894092
A_51_P100347 1.945398081
A_51_P100379 2.652902488
A_51_P100428 2.3705121

Total number of rows: 41174

Table truncated, full table size 999 Kbytes.

Supplementary file Size Download File type/resource
GSM1017584_US23502387_251486833881_S01_GE1-v5_95_Feb07_1_3.txt.gz 9.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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