|
Status |
Public on Aug 31, 2014 |
Title |
Liver, rep3 |
Sample type |
RNA |
|
|
Source name |
Liver
|
Organism |
Mus musculus |
Characteristics |
strain background: C57BL/6 tissue: Liver replicate: 3
|
Treatment protocol |
B cells were purified from spleen using CD19-microbeads (MB) (Miltenyi Biotec). Liver was dissected and washed in PBS then was snap frozen on dry ice.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared using the Rneasy kit (Qiagen).
|
Label |
cy3
|
Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to Agilent’s protocol.
|
|
|
Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
Description |
Sample5
|
Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.3.1 (Agilent) using default parameters (protocol GE1-v5_95_Feb07 and Grid: 014868_D_F_20060807) to obtain background subtracted and spatially detrended Processed Signal intensities.
|
|
|
Submission date |
Oct 10, 2012 |
Last update date |
Aug 31, 2014 |
Contact name |
Deepak Mav |
Organization name |
Sciome LLC
|
Street address |
2 Davis Drive
|
City |
Research Triangle Park |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
|
|
Platform ID |
GPL7202 |
Series (2) |
GSE41449 |
Epigenetic regulation of gene expression is instructed by promoter sequence and refined by developmental progression [expression] |
GSE41450 |
Epigenetic regulation of gene expression is instructed by promoter sequence and refined by developmental progression. |
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