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Sample GSM1017580 Query DataSets for GSM1017580
Status Public on Aug 31, 2014
Title B-cells, rep1
Sample type RNA
 
Source name B-cells
Organism Mus musculus
Characteristics strain background: C57BL/6
tissue: B-cells
replicate: 1
Treatment protocol B cells were purified from spleen using CD19-microbeads (MB) (Miltenyi Biotec). Liver was dissected and washed in PBS then was snap frozen on dry ice.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using the Rneasy kit (Qiagen).
Label cy3
Label protocol Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to Agilent’s protocol.
 
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slides were washed and then scanned with an Agilent Scanner.
Description Sample1
Data processing The scanned images were analyzed with Feature Extraction Software 9.5.3.1 (Agilent) using default parameters (protocol GE1-v5_95_Feb07 and Grid: 014868_D_F_20060807) to obtain background subtracted and spatially detrended Processed Signal intensities.
 
Submission date Oct 10, 2012
Last update date Aug 31, 2014
Contact name Deepak Mav
Organization name Sciome LLC
Street address 2 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL7202
Series (2)
GSE41449 Epigenetic regulation of gene expression is instructed by promoter sequence and refined by developmental progression [expression]
GSE41450 Epigenetic regulation of gene expression is instructed by promoter sequence and refined by developmental progression.

Data table header descriptions
ID_REF
VALUE The gProcessedSignal was log2-transformed and qunatile normalized to correct for between sample variation.

Data table
ID_REF VALUE
A_51_P100021 2.073255993
A_51_P100034 13.55313308
A_51_P100052 2.035405107
A_51_P100063 1.64581355
A_51_P100084 5.364224927
A_51_P100099 6.671481622
A_51_P100155 11.0928004
A_51_P100174 8.622637497
A_51_P100181 5.591963276
A_51_P100218 5.582927546
A_51_P100227 8.439118641
A_51_P100238 1.831775086
A_51_P100246 9.291225571
A_51_P100289 9.867023861
A_51_P100298 3.354853877
A_51_P100309 2.072302674
A_51_P100327 10.04553302
A_51_P100347 2.575053935
A_51_P100379 2.769879008
A_51_P100428 3.181058253

Total number of rows: 41174

Table truncated, full table size 999 Kbytes.




Supplementary file Size Download File type/resource
GSM1017580_US23502387_251486833708_S01_GE1-v5_95_Feb07_1_2.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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