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Sample GSM101187 Query DataSets for GSM101187
Status Public on Nov 15, 2006
Title Tup11-GFP ChIP-CHIP ORF/IGR E120D Sl6
Sample type genomic
 
Channel 1
Source name Tup11-GFP Chromatin Input
Organism Schizosaccharomyces pombe
Characteristics Tup11-GFP Input. Cells were grown to mid log phase (107cells/ml) in rich YES media. All cells were harvested at room temperature and subjected to crosslinking, sonication, extraction, amplification,labelling,hybridization,washing. scanning,quantification,normalisation and analysis as described previously (Robyr et al, 2003 and Wiren et al, 2005)
Extracted molecule genomic DNA
Label CY3
 
Channel 2
Source name Tup11-GFP Chromatin Immunoprecipitation
Organism Schizosaccharomyces pombe
Characteristics Tup11-GFP Chromatin Immunoprecipitation. Cells were grown to mid log phase (107cells/ml) in rich YES media. All cells were harvested at room temperature and immediately subjected to crosslinking, sonication, precipitation, extraction, amplification, labelling,hybridization,washing scanning,quantification,normalisation and analysis as described previously (Robyr et al, 2003 and Wiren et al, 2005)
Extracted molecule genomic DNA
Label CY5
 
 
Description Chromatin Immunoprecipitations.
Chromatin immunoprecipitations were essentially performed as described previously (Robyr et al, 2003, Wiren et al, 2005). Cells were grown to mid log phase (107cells/ml) in rich YES media. All cells were harvested at room temperature and immediately subjected to crosslinking. The lysate was subjected to sonication 3x60 sec for generation of chromatin fragments with appropriate length. The lysate from the two sonications was pooled, aliquoted and subjected to immunoprecipiation with a polyclonal a-GFP antibody 1:100 (Applied Biosciences 8372-2) and protein A sepharose beads (Amersham 17-5280-01). Chromatin bound beads were washed and eluated fromm beads. DNA was purified with phenol-chloroform extraction and ethanol-sodium acetate precipitation. Precipitated chromatin and input DNA fragments from three individually separated experiments were subjected to a two step linear amplification. Round A was performed with Sequenase (USB 70775Y) and TPCRA priming. Round B was performed with Amplitaq (Roche) with TPCRB priming. Amplified material (500-1000ng) was subjected to Klenow labelling (Invitrogen 18094-011) with Cy3 and Cy5 (Amersham 53021 and 55021). Generally the input fragments was labelled with Cy3 and precipitated material was labelled with Cy5. Reportedly minimal dye swap bias has previously been observed (Robyr et al, 2003) and labelling was therefore not carried out in dye swap. Labelled fragments were hybridized to IGR+ORF microarrays from Eurogentech SA, Seraing, Belgium. Microarray signals were visualized with ScanExpress laser scanner and quantified with the TIGR spotfinder quantification software. Data was analysed and median normalized to the 50th percentile with the GeneSpring software (Silicon Genetics) to generate six data points for each binding map. Flag values were subtracted from the data set and ratios were subjected to median percentile ranking analysis described (Buck et al, 2004). Cutoff values for the generation of binding lists were set to the 85th percentile.
Data processing Genespring Silicon Genetics
 
Submission date Mar 21, 2006
Last update date Jun 20, 2006
Contact name Anthony Wright
E-mail(s) yongtao.xue.franzen@ki.se
Organization name Karolinska Institute
Lab Anthony Wright
Street address Blickagången 6
City Huddinge
ZIP/Postal code 141 57
Country Sweden
 
Platform ID GPL3452
Series (2)
GSE4505 Tup11-GFP ChIP-CHIP
GSE4566 Role of transcriptional co-repressor proteins Ssn6, Tup11 and Tup12

Data table header descriptions
ID_REF
VALUE

Data table
ID_REF VALUE
1 0.28
2 0.36
3 1.44
4 -2.00
5 -0.12
6 -2.00
7 -2.00
8 -2.00
9 -2.00
10 -2.00
11 0.9
12 0.89
13 -2.00
14 -2.00
15 -2.00
16 -2.00
17 -2.00
18 -2.00
19 -2.00
20 -2.00

Total number of rows: 22176

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM101187.txt.gz 1.2 Mb (ftp)(http) TXT

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