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Status
Public on Nov 15, 2006
Title
Ssn6 Overexpression L040B sl7
Sample type
RNA
Channel 1 Source name
WT-pDUAL-Ssn6
Organism
Schizosaccharomyces pombe Characteristics
WT(FY367)-pDUAL-Ssn6 MM -URA 30 Deg
Treatment protocol
Xue et al, 2004
Growth protocol
Xue et al, 2004
Extracted molecule
total RNA
Extraction protocol
Xue et al, 2004
Label
CY3
Label protocol
Xue et al, 2004
Channel 2 Source name
WT-pDUAL-empty
Organism
Schizosaccharomyces pombe Characteristics
WT(FY367)-pDUAL-Empty MM-URA 30 Deg
Treatment protocol
Xue et al, 2004
Growth protocol
Xue et al, 2004
Extracted molecule
total RNA
Extraction protocol
Xue et al, 2004
Label
CY5
Label protocol
Xue et al, 2004
Hybridization protocol
Xue et al, 2004
Scan protocol
Scanexpress Laser scanner
Description
We have performed a genome wide expression profile study of the essential transcriptional co-repressor protein Ssn6 in Schizosaccharomyces pombe. Ssn6 affected targets were identified by over expression of Ssn6 protein in a WT strain. A WT strain holding a plasmid expressing Ssn6 (pDUAL-Ssn6) was compared against the expression in a WT with the empty plasmid (pDUAL-EMPTY) strain under selection for the plasmids MM-URA media at 30 degrees (Xue et al, 2004).
Data processing
Genespring Silicon Genetics Software
Submission date
Mar 21, 2006
Last update date
Jun 20, 2006
Contact name
Anthony Wright
E-mail(s)
yongtao.xue.franzen@ki.se Organization name
Karolinska Institute
Lab
Anthony Wright
Street address
Blickagången 6
City
Huddinge
ZIP/Postal code
141 57
Country
Sweden
Platform ID
GPL3454 Series (2)
GSE4502 Ssn6 Overexpression
GSE4566 Role of transcriptional co-repressor proteins Ssn6, Tup11 and Tup12
Data table header descriptions ID_REF VALUE Log2 of Normalised Ratio
Data table ID_REF VALUE 1
0.20
2
-0.01
3
-0.01
4
-0.04
5
0.88
6
-0.10
7
0.20
8
-0.08
9
0.23
10
-0.02
11
0.14
12
-0.05
13
-0.15
14
-0.35
15
0.05
16
-0.05
17
0.04
18
-0.16
19
-0.13
20
-0.07
11520 118 Kbytes .Supplementary file Size Download File type/resource GSM101139.txt.gz
342.3 Kb
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