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Sample GSM1006382 Query DataSets for GSM1006382
Status Public on Sep 20, 2012
Title Th2 42 hr Input
Sample type SRA
Source name Th2 polarized cells
Organism Mus musculus
Characteristics strain: C57BL/6
cell type/growth condition: Naïve T-cells from spleen under Th2 polarizing conditions
timepoint: 42 hrs
Extracted molecule genomic DNA
Extraction protocol 20000000 cells were harvested, crosslinked with formaldehyde, sonicated, IPed with appropriate antibodies and reverse-crosslinked to obtain 300-500 bp DNA fragments. These DNA fragments were ligated to Illumina TruSeq adapters for sequencing on the Illumina GAIIx.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
Data processing Illumina Casava1.7 software used for basecalling and generating eland extended files mapped onto mm9
Sequenced reads for the BMDCs were mapped to mm9 whole genome using gsnap with parameter s-d mm9 -Q -n 2 -A sam
QuEST was used for peak calling on the aligned eland extended or SAM files with the recommended 30 -fold enrichment used as threshold.
Genome_build: mm9
Supplementary_files_format_and_content: wig files indicating the reads aligned to the mm9 genome
Submission date Sep 19, 2012
Last update date May 15, 2019
Contact name Smita Agrawal
Organization name Genentech Inc.
Department Immunology
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
Platform ID GPL11002
Series (2)
GSE40483 A genomic regulatory element that directs assembly and function of immunespecific AP-1-IRF complexes
GSE40727 ChIPseq analysis of IRF4 and BATF in immune cells
SRA SRX188662
BioSample SAMN01179409

Supplementary file Size Download File type/resource
GSM1006382_Th2_42hr_background_normalized.profile.wig.gz 743.6 Kb (ftp)(http) WIG
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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