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Series GSE99921 Query DataSets for GSE99921
Status Public on Sep 20, 2018
Title Extensive sex differences at the initiation of meiotic recombination
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Third-party reanalysis
Methylation profiling by high throughput sequencing
Summary Meiotic recombination differs between males and females, however, when and how these differences are established is unknown. We identify extensive sex differences at recombination initiation by mapping hotspots of meiotic DNA double strand breaks in male and female mice. Contrary to past findings in humans, few hotspots are used uniquely in either sex. Instead, grossly different recombination landscapes result from up to 15-fold differences in hotspot use between males and females. Indeed, most recombination occurs at sex-biased hotspots. Sex biased hotspots appear to be partly determined by chromosome structure, and DNA methylation, absent in females at the onset of meiosis, plays a substantial role. Sex differences are also evident later in meiosis as the repair frequency of distal meiotic breaks as crossovers diverges in males and females. Suppression of distal crossovers may help to minimize age-related aneuploidy that arises due to cohesion loss during dictyate arrest in females.
Overall design Comparison of meiotic DNA double strand break hotspots in mouse testis and ovary
Contributor(s) Brick K, Thibault-Sennett S, Smagulova F, Lam KW, Pu Y, Pratto F, Camerini-Otero RD, Petukhova GV
Citation(s) 30185906
Submission date Jun 12, 2017
Last update date Jul 25, 2021
Contact name Kevin Brick
Organization name NIDDK
Department GBB
Street address 5/205 Memorial Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (3)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (22)
GSM2664274 Testis SSDS 200k cells
GSM2664275 Testis SSDS Sample 1
GSM2664276 Testis SSDS Sample 2
BioProject PRJNA390158
SRA SRP109024

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Supplementary file Size Download File type/resource
GSE99921_RAW.tar 61.1 Gb (http)(custom) TAR (of BAM, BED, BEDGRAPH)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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