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| Status |
Public on Jul 12, 2017 |
| Title |
Molecular pathogenesis of human prostate basal cell hyperplasia reveals a keratinocyte metaplasia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The incidence of basal cell hyperplasia (BCH) has been reported at 8-10%, but a molecular and cellular characterization has not been performed on this phenotype. Using freshly digested tissue from surgical specimens, we performed transcriptomic analysis of flow cytometry-purified basal epithelia from patients with and without a majority BCH phenotype. Gene set enrichment analysis revealed an increased expression of members of the epidermal differentiation complex, resembling the progression of other metaplastic diseases.
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| Overall design |
In order to generate a clean molecular profile of BCH, free of contaminating signal from leukocytes, stroma and luminal epithelia, we performed RNA sequencing on bulk-isolated basal epithelia from 3 patients with basal hyperplasia and 4 patients without BCH
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| Contributor(s) |
Strand DW, Henry GH |
| Citation(s) |
28795417 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K01 DK098277 |
Mechanisms of Fatty Acid Metabolism in Prostate Differentiation and Disease |
UT SOUTHWESTERN MEDICAL CENTER |
Douglas William Strand |
| R03 DK110497 |
Interplay Between Stem Cells and Inflammation in Benign Prostatic Hyperplasia |
UT SOUTHWESTERN MEDICAL CENTER |
Douglas William Strand |
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| |
| Submission date |
Jun 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Gervaise H. Henry |
| E-mail(s) |
gervaise.henry@utsouthwestern.edu
|
| Phone |
2146480497
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Urology
|
| Lab |
Strand Lab
|
| Street address |
5323 Harry Hines Blvd, Mail Code:9110
|
| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA389735 |
| SRA |
SRP108858 |
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