|Public on May 30, 2022
|The effect of dietary fibre exposure on gene expression profiles of THP-1 macrophages
|Expression profiling by array
|Background: Macrophages represent an important part of the immune system in the intestine and are crucial for maintaining homeostasis. As part of research investigating the effect of dietary fibres on the intestinal immune barrier THP-1 macrophages were used as model system. Methods: THP-1 monocytes were stimulated for 48 hours with 100 ng/ml PMA and 48 hours rested in medium. Subsequently, they were stimulated with 500 ug/ml dietary fibres and the maximal observed LPS contamination to serve as background control. After 6 hours, total RNA was extracted and Affymterix Human Gene 1.1 ST arrays were used to analyze the gene expression profiles. To identify dietary fibre induced gene expression profiles in dietary fibre gene responses were compared to medium samples. Furthermore, to analyse differentiatlly affected pathways Ingenuite Pathway Analysis was employed. Results: There was a clear difference in significantly differentially expressed genes (gene cut-off p<0.05) with beta-glucan oat medium viscosity and GOS changing transcription of a relative small amount of genes and Sugar beet pectin and Resistant starch a relative large amount of genes. These latter two were also the only dietary fibres to demonstrate an increase in Fc-receptor-related pathway activation. Alternatively, beta-glucan oat medium viscosity and GOS were the only dietary fibres to activate pathways related to cellular movement and the only two to not activate the Ahr-signaling pathway (p<0.05). Conclusion: our data indicate that the in vitro THP-1 macrophage model can be used to differentiate in immunomodulatory potential of dietary fibres and provide hypotheses for functional differentiation.
|Microarray analysis was performed on THP-1 macrophages incubated with dietary fibres for 6 hours. This experiment was performed three times, so three independent biological replicates per treatment were included in the microarray analysis.
|Govers C, Mes JJ, Boekschoten MV, de Vos P
|Jun 02, 2017
|Last update date
|Jun 01, 2022
|Wageningen Food & Biobased Research
|Bornse Weilanden 9
|[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
|This SubSeries is part of SuperSeries:
|The effect of dietary fibre exposure on gene expression profiles in Caco-2 and THP-1 cells