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Series GSE99611 Query DataSets for GSE99611
Status Public on May 30, 2022
Title The effect of dietary fibre exposure on gene expression profiles of THP-1 macrophages
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background: Macrophages represent an important part of the immune system in the intestine and are crucial for maintaining homeostasis. As part of research investigating the effect of dietary fibres on the intestinal immune barrier THP-1 macrophages were used as model system. Methods: THP-1 monocytes were stimulated for 48 hours with 100 ng/ml PMA and 48 hours rested in medium. Subsequently, they were stimulated with 500 ug/ml dietary fibres and the maximal observed LPS contamination to serve as background control. After 6 hours, total RNA was extracted and Affymterix Human Gene 1.1 ST arrays were used to analyze the gene expression profiles. To identify dietary fibre induced gene expression profiles in dietary fibre gene responses were compared to medium samples. Furthermore, to analyse differentiatlly affected pathways Ingenuite Pathway Analysis was employed. Results: There was a clear difference in significantly differentially expressed genes (gene cut-off p<0.05) with beta-glucan oat medium viscosity and GOS changing transcription of a relative small amount of genes and Sugar beet pectin and Resistant starch a relative large amount of genes. These latter two were also the only dietary fibres to demonstrate an increase in Fc-receptor-related pathway activation. Alternatively, beta-glucan oat medium viscosity and GOS were the only dietary fibres to activate pathways related to cellular movement and the only two to not activate the Ahr-signaling pathway (p<0.05). Conclusion: our data indicate that the in vitro THP-1 macrophage model can be used to differentiate in immunomodulatory potential of dietary fibres and provide hypotheses for functional differentiation.
Overall design Microarray analysis was performed on THP-1 macrophages incubated with dietary fibres for 6 hours. This experiment was performed three times, so three independent biological replicates per treatment were included in the microarray analysis.
Contributor(s) Govers C, Mes JJ, Boekschoten MV, de Vos P
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Submission date Jun 02, 2017
Last update date Jun 01, 2022
Contact name Coen Govers
Phone +31 317482850
Organization name Wageningen Food & Biobased Research
Street address Bornse Weilanden 9
City Wageningen
State/province N/A
ZIP/Postal code 6708 WG
Country Netherlands
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (24)
GSM2648567 G114_F01_61_THP-1_exp1_medium
GSM2648568 G114_F02_62_THP-1_exp1_LPS
GSM2648569 G114_F03_63_THP-1_exp1_Novelose
This SubSeries is part of SuperSeries:
GSE99612 The effect of dietary fibre exposure on gene expression profiles in Caco-2 and THP-1 cells
BioProject PRJNA388898

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99611_RAW.tar 113.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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