|Public on May 30, 2022
|The effect of dietary fibre exposure on gene expression profiles in intestinal Caco-2 cells
|Expression profiling by array
|Background: Human intestinal tissue samples are barely accessible to study potential health benefits of nutritional compounds. Dietary fibres have been descirbed to be beneficial for intestinal health. Therefore, in this study we explored the applicability of an in vitro model, namely human intestinal Caco-2 cells, to study the effect of dietary fibres on intestinal health. Transcriptomics was applied to obtain more insight into their mode of actions in the intestinal cells. Methods: Caco-2 cells were stimulated with 500 ug/ml dietary fibres and the maximal observed LPS contamination to serve as background control for 6 hours, total RNA was extracted and Affymterix Human Gene 1.1 ST arrays were used to analyze the gene expression profiles. To identify dietary fibre induced gene expression profiles in dietary fibre gene responses were compared to medium samples. Furthermore, to analyse differentiatlly affected pathways Ingenuite Pathway Analysis was employed. Results: Pathway analysis revealed a distinct separation between the dietary fibres. GOS and beta-glucan oat medium viscosity affected transcription of a lower amount of genes (gene cut-off p<0.05) and gen transcription changes suggest an increase in vesicle transport and altered cholesterol regulation. On the other hand, the other dietary fibres differentially regulated a larger numbers of genes (gene cut-off p<0.05) and all appeared related to immune responses. We observed an increase in intracellular and extracellular anti-bacterial pathways and production of cytokines specifically aimed at communication with the adaptive immune system. Conclusion: GOS and beta-glucan oat medium viscosity appeared to induce intestinal epithelial communication with the body, whereas the other dietary fibres appeared recognized as PAMP and induce epithelial cells to interact with the immune system.
|Microarray analysis was performed on 21-days differentiated Caco-2 cells incubated with dietary fibres and LPS and medium control for 6 hours. This experiment was performed three times, so three independent biological replicates per treatment were included in the microarray analysis.
|Govers C, Mes JJ, Boekschoten MV, de Vos P
|Jun 02, 2017
|Last update date
|Jun 01, 2022
|Wageningen Food & Biobased Research
|Bornse Weilanden 9
|[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
|This SubSeries is part of SuperSeries:
|The effect of dietary fibre exposure on gene expression profiles in Caco-2 and THP-1 cells