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Series GSE99289 Query DataSets for GSE99289
Status Public on May 26, 2017
Title Human AsPC1-CFP and AsPC1-AKT PDAC orthotopic tumors: Lean vs Obese
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Transcriptional profiling comparing human AsPC1 PDA cells transduced with either CFP control or myristoylated-AKT orthotopically grown in pancreata of lean vs obese Rag1-/- immunodeficient mice for 5 weeks (100,000 cells/pancreas). The Goal was to determine the transcriptional deregulation of Obesity-driven and AKT-driven tumors.
Overall design Four-condition experiment: AsPC1-CFP lean, AsPC1-CFP obese, AsPC1-AKT lean and AsPC1-AKT obese tumors all normalized to one control: AsPC1-CFP cells. 9 biological replicates per group. Equal amounts of differentially labeled control AsPC1-CFP cells propagated in culture and sample tumor cRNA were combined such that each sample contained at least 2.5 pmol dye on dual-labeling arrays.
Contributor(s) Kalaany N, Zaytouni T
Citation(s) 28808255
Submission date May 25, 2017
Last update date Jul 25, 2021
Contact name Nada Y Kalaany
Organization name Boston Children's Hospital
Street address 3 Blackfan Circle CLS 16067
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (36)
GSM2640510 AsPC1-CFP lean tumor replicate 1
GSM2640511 AsPC1-CFP lean tumor replicate 2
GSM2640512 AsPC1-CFP lean tumor replicate 3
BioProject PRJNA388041

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99289_RAW.tar 217.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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