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Series GSE99272 Query DataSets for GSE99272
Status Public on Apr 21, 2019
Title Integrative epigenomic analyses of early-life hypothalamic response to augmented maternal care [BiSulfite-seq]
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary The quality of maternal care in early-life plays a crucial role in mammalian neurodevelopment. Augmented maternal care (AMC) is a well-established rodent model of enhanced neonatal care. Rats that have undergone AMC have improved stress resilience and cognition compared with rats that have experienced normal levels of maternal care or adverse neonatal stress. However, the epigenomic basis of long-lived responses to AMC has not been previously explored. Thus, we employed whole-genome bisulfite sequencing (WGBS), RNA-sequencing (RNA-seq), and a multiplex microRNA (miRNA) assay to assess DNA cytosine methylation, gene expression, and miRNA expression, respectively. The integrated results identify a suite of 20 prioritized candidates impacted by AMC. Overall, these results identified AMC-induced regulatory differences in genes related to neurotransmission, neurodevelopment, protein synthesis, and oxidative phosphorylation in addition to the expected stress response genes. Together, these unbiased results represent a key progression in understanding the complex mechanisms underlying the early-life mechanisms for AMC programming stress resiliency.
Overall design DNA methylation and RNA were assayed in augmented maternal care male rats as well as controls.
Contributor(s) Dunaway K, Yasui D, Ciernia AV, Laufer B
Citation(s) 29613827
Submission date May 24, 2017
Last update date Jul 25, 2021
Contact name Keith Dunaway
Organization name UC Davis
Department MMI
Lab LaSalle Lab
Street address 3318 Tupper Hall
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (6)
GSM2640261 AMC1_WGBS
GSM2640262 AMC2_WGBS
GSM2640263 AMC3_WGBS
This SubSeries is part of SuperSeries:
GSE99282 Integrative epigenomic analyses of early-life hypothalamic response to augmented maternal care
BioProject PRJNA387915
SRA SRP107963

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Supplementary file Size Download File type/resource
GSE99272_RAW.tar 806.5 Mb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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