GEO help: Mouse over screen elements for information.
|Public on Jun 25, 2018
|Expression data from ovarian cells growing in three dimensional culture (Matrigel) with altered expression of miR-200s
|Expression profiling by array
|It is believed that incessant ovulation will cause formation of ovarian cysts which later lead to ovarian cancer maglinancy. miR-200s is highly expressed in ovarian cancer tissue when compared to ovarian normal tissue. To study wether miR-200s have a pathologenic role in ovarian cyst formation, normal ovarian cells with overexpression of miR-200s and two cancer cell lines with knockdown of miR-200s were grown in a 3D culture system and allowed to form cyst.
We used microarrays to detail the global programme of gene expression underlying ovarian cyst formation affected by expression of miR-200s and indentify distinct classes of up-regulated and down-regulated genes during this process.
|Normal ovarian surface epithelial cells (OSE7) with overexpression of miR-200s and ovarian cancer cells (MCAS and OVCA432) with knockdown of miR-200s growing in Matrigel for seven days were subjected to RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the gene expression altered by miR-200s level after the cells encounted Matrigel environment for 7 days, when cysts are usually formed. miR-200s consists of five members (miR-200a, miR-200b, miR-200c, miR-141 and miR-429). These five miRNAs form two clusters: cluster 1 of miR-200b, miR-200a and miR-429 maps to chromosome 1 (1p33.36), while the miR-200c and miR-141 cluster maps to chromosome 12 (12p13.31). Alternatively, the miR-200 family members can also be categorized in two functional groups based upon the similarities of their seed sequences. MiR-200b, miR-200c and miR-429 (Functional Group I) all share the same seed sequence (5’-AAUACUG-3’), while miR-200a and miR-141 (Functional Group II) both share the same seed sequence (5’-AACACUG-3’), with the two functional groups differing in the seed sequence only by one nucleotide. MiR-200s were over-expressed or knockdown in ovarian cells according to the dichotomous location rather than to their seed sequence because the qRT-PCR data from our previous work on miR-200 in ovarian cells showed an obvious dichotomous expression pattern of the miR-200 family members according to chromosome location. We used lentivrus system to overexpress or knockdown the genes. Scramble control were established with the lentiviral vector inserted with a random sequence for both normal and cancer cell lines.
|Choi P, Liu S, Wing N
|May 23, 2017
|Last update date
|Jul 25, 2021
|pui wah Choi
|Brigham and Women's Hospital
|GYN oncology, Alex Ng
|221 longwood Ave
|[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
|Wild type OSE7
|OSE7 transduced by virus carry vector inserted with a random sequence
|OSE7 with miR-200 cluster 1 overexpressed
|TAR (of CEL)