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Series GSE99217 Query DataSets for GSE99217
Status Public on Jun 25, 2018
Title Expression data from ovarian cells growing in three dimensional culture (Matrigel) with altered expression of miR-200s
Organism Homo sapiens
Experiment type Expression profiling by array
Summary It is believed that incessant ovulation will cause formation of ovarian cysts which later lead to ovarian cancer maglinancy. miR-200s is highly expressed in ovarian cancer tissue when compared to ovarian normal tissue. To study wether miR-200s have a pathologenic role in ovarian cyst formation, normal ovarian cells with overexpression of miR-200s and two cancer cell lines with knockdown of miR-200s were grown in a 3D culture system and allowed to form cyst.
We used microarrays to detail the global programme of gene expression underlying ovarian cyst formation affected by expression of miR-200s and indentify distinct classes of up-regulated and down-regulated genes during this process.
Overall design Normal ovarian surface epithelial cells (OSE7) with overexpression of miR-200s and ovarian cancer cells (MCAS and OVCA432) with knockdown of miR-200s growing in Matrigel for seven days were subjected to RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the gene expression altered by miR-200s level after the cells encounted Matrigel environment for 7 days, when cysts are usually formed. miR-200s consists of five members (miR-200a, miR-200b, miR-200c, miR-141 and miR-429). These five miRNAs form two clusters: cluster 1 of miR-200b, miR-200a and miR-429 maps to chromosome 1 (1p33.36), while the miR-200c and miR-141 cluster maps to chromosome 12 (12p13.31). Alternatively, the miR-200 family members can also be categorized in two functional groups based upon the similarities of their seed sequences. MiR-200b, miR-200c and miR-429 (Functional Group I) all share the same seed sequence (5’-AAUACUG-3’), while miR-200a and miR-141 (Functional Group II) both share the same seed sequence (5’-AACACUG-3’), with the two functional groups differing in the seed sequence only by one nucleotide. MiR-200s were over-expressed or knockdown in ovarian cells according to the dichotomous location rather than to their seed sequence because the qRT-PCR data from our previous work on miR-200 in ovarian cells showed an obvious dichotomous expression pattern of the miR-200 family members according to chromosome location. We used lentivrus system to overexpress or knockdown the genes. Scramble control were established with the lentiviral vector inserted with a random sequence for both normal and cancer cell lines.
Contributor(s) Choi P, Liu S, Wing N
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Submission date May 23, 2017
Last update date Jul 25, 2021
Contact name pui wah Choi
Phone 6173563166
Organization name Brigham and Women's Hospital
Department OB/GYB
Lab GYN oncology, Alex Ng
Street address 221 longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (13)
GSM2636222 Wild type OSE7
GSM2636223 OSE7 transduced by virus carry vector inserted with a random sequence
GSM2636224 OSE7 with miR-200 cluster 1 overexpressed
BioProject PRJNA387584

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99217_RAW.tar 56.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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