 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 23, 2017 |
| Title |
Plasma MicroRNA alterations between EGFR-activating mutational NSCLC patients with and without primary resistance to TKI |
| Platform organisms |
Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Macacine alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Herpesvirus saimiri (strain 11); JC polyomavirus; Human immunodeficiency virus 1; Human betaherpesvirus 6B; Human gammaherpesvirus 8; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
The aim of the present study was to examine differentially expressed miRNAs in plasma between EGFR-TKIs sensitive and EGFR-TKIs primary resistance patients.
|
| |
|
| Overall design |
MiRNA microarray of plasma from patients’ blood identified 16 differentially expressed miRNAs of which 15 (hsv2-miR-H19, hsa-miR-744-5p, hsa-miR-3196, hsa-miR-3153, hsa-miR-4791, hsa-miR-4803, hsa-miR-4796-3p, hsa-miR-372-5p, hsa-miR-138-2-3p, hsa-miR-16-1-3p, hsa-miR-1469, hsa-miR-585-3p, ebv-miR-BART14-5p, hsa-miR-769-3p, hsa-miR-548aq-5p) were down regulated while only hsa-miR-503-3p was up regulated in primary resistant patients’ plasma. Volcano plot and hierarchical clustering were performed to examine the accuracy of the miRNAs. Then validation with quantitative real-time PCR was performed and the result was in accordance with the array data. Functional analysis of these differentially expressed miRNAs with Ingenuity Pathway Analysis (IPA) revealed a common signaling network including MYC, CCND1, IGF1 and RELA.
|
| |
|
| Contributor(s) |
Pan X, An H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 22, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hanxiang An |
| E-mail(s) |
174604381@qq.com
|
| Phone |
18805921300
|
| Organization name |
xiamen cancer hospital
|
| Street address |
siming district Zhenhai road 55#, 1st affiliated hospital of xiamen university
|
| City |
XIAMEN |
| State/province |
Fujian |
| ZIP/Postal code |
361000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL21439 |
miRCURY LNA microRNA Array, 7th generation - hsa, mmu & rno (miRBase 21; probeID version) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA387425 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99143_RAW.tar |
7.7 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...