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Series GSE99076 Query DataSets for GSE99076
Status Public on May 19, 2017
Title Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To elucidate the molecular bases for the infertile phenotype of Zmym3 KO mice at the mRNA expression level, we carried out RNA sequencing (RNA-seq) analysis on cultured spermatogonial stem cells (SSCs), c-KIT+ pre-meiotic cells induced from SSCs and spermatocytes directly isolated from adult mouse testes.
Overall design The cells used for RNA-Seq analysis including SSCs from Zmym3 WT and KO mice (SSC:WT and SSC:KO), c-KIT+ pre-meiotic cells induced from SSCs (c-KIT+ WT and c-KIT+:KO) and spermatocytes from 5- and 7-month mice (5m-SC:WT, 5m-SC:KO, 7m-SC:WT and 7m-SC:KO).
Contributor(s) Hu X, Lin X, Han C
Citation(s) 28661483
Submission date May 18, 2017
Last update date Jul 25, 2021
Contact name Chunsheng Han
Phone 86-10-64807105
Organization name Chinese Academy of Sciences
Department Institute of Zoology
Lab State Key Laboratory of Reproductive Biology
Street address 1-5 Beichen West Road, Chaoyang District
City Beijing
State/province Beijing
ZIP/Postal code 100101
Country China
Platforms (2)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (12)
GSM2631946 Zmym3 WT SSC replicate 1
GSM2631947 Zmym3 WT SSC replicate 2
GSM2631948 Zmym3 KO SSC replicate 1
BioProject PRJNA387186
SRA SRP107342

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Supplementary file Size Download File type/resource
GSE99076_RAW.tar 8.3 Mb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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