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| Status |
Public on Dec 15, 2007 |
| Title |
Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have studied the plasma membrane protein phenotype of human culture-amplified and native Bone Marrow Mesenchymal Stem Cells (BM MSCs). We have found, using microarrays and flow cytometry, that cultured cells express specifically 113 transcripts and 17 proteins that were not detected in hematopoietic cells. These antigens define a lineage-homogenous cell population of mesenchymal cells, clearly distinct from the hematopoietic lineages, and distinguishable from other cultured skeletal mesenchymal cells (periosteal cells and synovial fibroblasts). Among the specific membrane proteins present on cultured MSCs, 9 allowed the isolation from BM mononuclear cells of a minute population of native MSCs. The enrichment in Colony-Forming Units-Fibroblasts was low for CD49b, CD90 and CD105, but high for CD73, CD130, CD146, CD200 and integrin alphaV/beta5. Additionally, the expression of CD73, CD146 and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in depth studies.
Keywords: cell type comparison
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| Overall design |
Expression profiles of bone marrow MSC were compared with different lineages of purified hematopoietic cells from bone marrow to identify characteristic markers for human BM-MSC
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| Contributor(s) |
Delorme B, Ringe J, Gallay N, LeVern Y, Kerboeuf D, Jorgensen C, Rosset P, Sensebé L, Layrolle P, Häupl T, Charbord P |
| Citation(s) |
18086871 |
| Submission date |
Dec 14, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Thomas Häupl |
| E-mail(s) |
thomas.haeupl@charite.de
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| Phone |
+49 30 450513293
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| Organization name |
Charité
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| Department |
Rheumatology
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| Street address |
Charitéplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA103891 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9894_RAW.tar |
66.4 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
| Processed data included within Sample table |
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