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| Status |
Public on May 12, 2017 |
| Title |
Gene expression analysis in whole blood samples obtained from donors diagnosed with MDD compared to healthy controls |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This study investigated gene expression changes in whole blood samples obtained from donors diagnosed with major depressive disorder (MDD) compared to healthy controls. Micro-array data were available from whole blood on patients with MDD (N=128, 64 with generalised anxiety disorder, diagnosed by the MINI questionnaire, and 64 without anxiety disorder) and healthy controls (N=64). RNA was isolated from all samples using the standard PAXgene protocol on the Qiagen Biorobot 8000. All samples gave good quality RNA, as assessed by Agilent Bioanalyser. The yield range was 0.86-15.05ug with an average of 6.25ug. Samples were then randomised into batches, with each batch containing a representative number of controls, depression with anxiety and depression without anxiety, and the same ratio of females to males (3:1). 50ng of RNA from each sample was converted to a biotin labeled cDNA probe using NuGEN SPIA amplification. The probes were then hybridized to Affymetrix U133_Plus2.0 Genechips.
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| Overall design |
Measurements from whole blood samples for 128 patients with MDD (among which 64 have been diagnosed with generalised anxiety disorder) and 64 healthy controls
contributor: MRC ImmunoPsychiatry Consortium/GlaxoSmithKline
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| Contributor(s) |
Kelly FM, Leday GG, Vértes PE, Richardson S, Greene JR, Regan T, Khan S, Henderson RB, Freeman TC, Pariante CM, Harrison NA, Perry HV, Devrets WC, Wittenberg GM, Bullmore ET |
| Citation(s) |
28688579 |
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| Submission date |
May 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Gwenael G.R. Leday |
| E-mail(s) |
gwenael.leday@mrc-bsu.cam.ac.uk
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| Organization name |
University of Cambridge
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| Department |
School of Clinical Medicine
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| Lab |
MRC Biostatistics Unit
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| Street address |
Forvie Site, Robinson Way
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0SR |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (192)
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| Relations |
| BioProject |
PRJNA386248 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE98793_RAW.tar |
857.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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