NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE98723 Query DataSets for GSE98723
Status Public on Sep 15, 2017
Title The Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb group proteins are transcriptional repressors that control cell identity and development. In mammals, at least five different CBX proteins are believed to associate with the core Polycomb repressive complex 1 (PRC1). CBX6 and CBX7 are the most highly expressed CBX proteins in embryonic stem cells (ESCs), yet little is known about the function of CBX6 in these cells. Here we show that CBX6 is an essential regulator of ESC identity, since ablation of CBX6 function destabilizes the pluripotency network and triggers differentiation. At the molecular level, CBX6 is linked to the canonical PRC1 (cPRC1) complex; however, contrary to expectations, our results indicate that CBX6 also has a non-canonical PRC1 function. Taken together, our findings reveal that CBX6 is an essential component for ESC biology and contributes to the structural and functional complexity of the PRC1 complex.
 
Overall design List of ChIPseq samples (Cbx6/Cbx7/Ring1b) and RNAseq samples (control and Cbx6 knock-down)
 
Contributor(s) Santanach A, Blanco E, DiCroce L
Citation(s) 29089522
Submission date May 09, 2017
Last update date Jul 25, 2021
Contact name Enrique Blanco
E-mail(s) enrique.blanco@crg.eu
Phone +34 93 316 01 00
Organization name Center for Genomic Regulation (CRG)
Department Gene Regulation, Stem Cells and Cancer
Lab Epigenetic Events in Cancer (L. Di Croce's lab)
Street address Dr. Aiguader 88
City Barcelona
ZIP/Postal code 08003
Country Spain
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (11)
GSM2610616 ChIP_HA-Cbx6_R1
GSM2610617 ChIP_HA-Cbx6_R2
GSM2610618 ChIP_HA-Cbx6_Control
Relations
BioProject PRJNA386280
SRA SRP106864

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98723_RAW.tar 1.2 Gb (http)(custom) TAR (of BED, BEDGRAPH)
GSE98723_RPKMs_shCtlR1R2-shCbx6R1R2.txt.gz 348.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data provided as supplementary file
Processed data are available on Series record
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap