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Series GSE98713 Query DataSets for GSE98713
Status Public on Feb 28, 2018
Title MicroRNAs-wide analysis of primordial germ and somatic cells reveals sexual and developmental differential functional regulation in mouse embryonic gonads
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary We have characterized by small-RNAseq the miRNA expression pattern of mouse male and female Primordial Germ Cells (PGCs) and somatic stromal cells from gonads at E11.5, E12.5 and E13.5. MiRNA accumulation was higher in somatic cells than in PGCs and more stable across the different developmental stages analyzed. Differential expression analyses showed differences in the regulation of key miRNA clusters such as miR-199-214, miR-182-183-96 and miR-34c-5p whose targets have defined roles in both germ and somatic cells in gonadal sexual determination. Extensive analyses of miRNA sequences revealed an increase in non-canonical isoforms in PGCs at E12.5 compared to E11.5 and E13.5 and a dramatic change in isomiR expression and non-template 3' nucleotide additions in female PGCs at E13.5 respect to the other samples.
Overall design miRNA profiles of 12 different mice samples, corresponding to PGCs and gonadal somatic cells from E11.5, E12.5 and E13.5 male and female gonads, generated by deep sequencing using MiSeq Sequencing System (Illumina).
Contributor(s) Fernandez-Perez D, Brieño-Enríquez MA, Isoler-Alcaraz J, Larriba E, del Mazo J
Citation(s) 29187591, 35750721
Submission date May 09, 2017
Last update date Jul 07, 2022
Contact name Jesus del Mazo Martinez
Organization name Centro de Investigaciones Biologicas (CIB) - CSIC
Street address C/ Ramiro de Maeztu 9
City Madrid
ZIP/Postal code 28040
Country Spain
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (12)
GSM2610529 PGC11F
GSM2610530 PGC11M
GSM2610531 PGC12F
BioProject PRJNA385972
SRA SRP106711

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Supplementary file Size Download File type/resource
GSE98713_All_samples_NormalizedCounts_DESeq.txt.gz 41.1 Kb (ftp)(http) TXT
GSE98713_RAW.tar 80.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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