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| Status |
Public on Dec 12, 2007 |
| Title |
Summarizing probe levels of Affymetrix arrays taking into account day-to-day variability |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Day-to-day variability in microarray experiments is a recognized source of variation that can impede the analysis of large microarray studies where samples are processed on different days. In this study, we have applied an algorithm, called D2Dsum, which is based on a log-linear fixed effect model to cope with this kind of issues on a data set of 45 microarrays.
Keywords: time course
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| Overall design |
A2780 human ovarian cancer cell lines have been treated (TRT) with two different chemical compounds (A and B) or left untreated (CTRL). RNA was collected form 1 to 6 hour every hour. For each time point a technical duplicate or triplicate has been performed. Samples were subjected to complete randomisation experimental design and the effect of hybridisation day has been evaluated.
processed on 4 different days
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| Contributor(s) |
Magni P, Simeoni A, Healy S, Isacchi A, Bosotti R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 10, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Roberta Bosotti |
| E-mail(s) |
roberta.bosotti@nervianoms.com
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| Organization name |
Nerviano Medical Sciences srl
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| Department |
Biotechnology
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| Lab |
Genomics
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| Street address |
viale Pasteur,10
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| City |
Nerviano |
| State/province |
MI |
| ZIP/Postal code |
20014 |
| Country |
Italy |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA103791 |
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