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Series GSE982 Query DataSets for GSE982
Status Public on Jan 30, 2004
Title Gene Expression-Based High Throughput Screening: HL-60 Cell Treatment with Candidate Compounds
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We developed a general approach to small molecule library screening called GE-HTS (Gene Expression-Based High Throughput Screening) in which a gene expression signature is used as a surrogate for cellular states and applied it to the identification of compounds inducing the differentiation of acute myeloid leukemia cells. In screening 1,739 compounds, we prioritized 15 candidate compounds (2 were already confirmed in the literature). We next evaluated the 13 remaining compounds. Eight reliably induced the differentiation signature, and furthermore yielded functional evidence of bona fide differentiation.

This data set contains HL-60 cells treated in replicates of 3 with the original 13 selected candidates. It also contains 6 untreated, 6 DMSO treated, 3 ATRA treated, 3 PMA treated, and 3 1,25-dihydroxyvitamin D3 treated HL-60 controls. In addition, it contains 3 neutrophil and 3 monocyte samples from distinct normal human donors and 9 primary patient AML samples. This data set was used to evaluate the whole genome effects of the candidate compounds on HL-60 cells.
Keywords = AML
Keywords = leukemia
Keywords = HL-60
Keywords = chemical genomics
Keywords: repeat sample
Contributor(s) Stegmaier K, Ross KN, Colavito SA, O'Malley S, Stockwell BR, Golub TR
Citation(s) 14770183
Submission date Jan 21, 2004
Last update date Aug 10, 2018
Contact name Ken Ross
Phone 617-714-7473
Fax 617-714-8955
Organization name Broad Institute
Department Cancer Genomics
Lab Golub Lab
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (75)
GSM15421 AML1
GSM15422 AML2
GSM15423 AML3
This SubSeries is part of SuperSeries:
GSE995 Differentiation of acute myeloid leukemia cells
BioProject PRJNA89873

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE982_RAW.tar 256.2 Mb (http)(custom) TAR (of CEL)

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