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| Status |
Public on Apr 17, 2019 |
| Title |
De novo sequencing of transcriptome of Sf9 cells after Bm-15 overexpression and repression |
| Organism |
Spodoptera frugiperda |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Small nucleolar RNAs (snoRNA) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs. But we found that knock down of a C/D box snoRNA, Bm-15, can induce apoptosis of insect Spodoptera frugiperda Sf9 cells. For the genome sequence of Spodoptera frugiperda is incomplete, here with the de novo sequencing method, transcriptome of Spodoptera frugiperda cell line Sf9 were sequenced after being transfected with overexpression vector and repression probes of snoRNA Bm-15. Results showed that 21 apoptosis-related genes were up-regulated upon Bm-15 inhibition and down-regulated with Bm-15 overexpression.
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| |
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| Overall design |
Examination of transcriptome of 3 different samples, normal Sf9 cells, cells with Bm-15 overexpression and repression.
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| Contributor(s) |
Li D, Kan Y |
| Citation(s) |
30971200 |
| |
| Submission date |
Apr 25, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Dandan Li |
| E-mail(s) |
lidannytc@126.com
|
| Phone |
86-13781771513
|
| Organization name |
Nanyang Normal University
|
| Lab |
Henan Key Laboratory of Insect Biology in Funiu Mountain
|
| Street address |
1638 Wolong Road
|
| City |
Nanyang |
| State/province |
Henan |
| ZIP/Postal code |
86-473061 |
| Country |
China |
| |
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| Platforms (1) |
| GPL17152 |
Illumina HiSeq 2000 (Spodoptera frugiperda) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA384239 |
| SRA |
SRP105214 |
