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| Status |
Public on Apr 25, 2018 |
| Title |
Loss of Dnmt3a Immortalizes Hematopoietic Stem Cells in vivo [WGBS] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Recent epidemiological studies have shown that cancer-associated DNMT3A mutations can be detected in the blood of a large proportion of asymptotic elderly individuals. It is important to understand how these mutations provide a competitive advantage to HSCs and allow them to establish clonal dominance. Here we demonstrate that the stress of serial HSC transplantation immortalizes Dnmt3a-null HSCs.
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| Overall design |
Dnmt3a-null system cells transplanted multiple times in mice were sequenced for RNA-Seq, ChIP-Seq, and methylation using Illumina Nextseq
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| Contributor(s) |
Park H, Jeong M, Goodell M, Challen G |
| Citation(s) |
29617651 |
| |
| Submission date |
Apr 25, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hyun Jung Park |
| E-mail(s) |
hjpark@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Dan L. Duncan Cancer Center
|
| Lab |
Li Lab
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE98191 |
Loss of Dnmt3a Immortalizes Hematopoietic Stem Cells in vivo |
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| Relations |
| BioProject |
PRJNA384233 |
| SRA |
SRP105210 |
