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Series GSE98143 Query DataSets for GSE98143
Status Public on Jun 20, 2017
Title Differential impact of Dicer deficiency on microglia of the developing and adult brain (Agilent miRNA microarrays)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Microglia seed the embryonic neuro-epithelium, expand and actively sculpt neuronal circuits in the developing CNS, but eventually adopt relative quiescence and ramified morphology in the adult. Here we probed the impact of post-transcriptional control by microRNAs (miRNAs) on microglial performance during development and adulthood by generating mice lacking microglial dicer expression at these distinct stages. RNA seq of conditional dicer ablation in adult microglia revealed that miRNAs were required to limit microglial responses to challenge but had only minor changes on non-challenged microglia. Specifically, following peripheral endotoxin exposure of the animals (LPS), Dicer-deficient microglia either from total brain or hippocampus overexpressed pro-inflammatory cytokines and as a result compromised hippocampal neuronal functions. In contrast, prenatal ablation resulted in spontaneous microglia activation and revealed Dicer involvement in DNA repair and preservation of genome integrity. Overall, our study shows miRNA and Dicer regulation of inflammatory response either following challenge in the adult or spontaneously in the newborn microglia. Moreover, Dicer is required for preservation of microglia DNA integrity required for their proliferation, longevity and radioresistance.
 
Overall design Adult Microglia (defined as CD45intCD11b+F4/80+Ly6C-Ly6G- cells, n=2, each replicte is a pool of 4-6 mice), Colon MFs (defined as CD45+CD11b+CD11c+CD64+Ly6C-, n=2, each replicate is a pool of 4-6 mice), and Liver MFs (KCs) (defined as CD45+CD11b+F4/80+CD64+MHCII+, n=2, each replicate is a pool of 4-6 mice) were sorted from 6-8 weeks old WT mice. miRNA microarray profiling was performed on the populations above in order to identify miRNAs enriched in steady state adult microglia.
 
Contributor(s) Varol D, Mildner A, David E, Jung S
Citation(s) 28636953
Submission date Apr 25, 2017
Last update date Jul 25, 2021
Contact name Steffen Jung
E-mail(s) s.jung@weizmann.ac.il
Phone 0097289342787
Organization name The Weizmann Institute of Science
Department Immunology and Regenerative Biology
Lab Steffen Jung
Street address Herzl
City REHOVOT
ZIP/Postal code 76100
Country Israel
 
Platforms (1)
GPL17912 Agilent-046065 Mouse_miRNA_V19.0_Microarray (miRNA ID version)
Samples (6)
GSM2588518 WT_MG_Rep1
GSM2588519 WT_MG_Rep2
GSM2588520 WT_Colon_MF_Rep1
This SubSeries is part of SuperSeries:
GSE98144 Differential impact of Dicer deficiency on microglia of the developing and adult brain
Relations
BioProject PRJNA384123

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98143_RAW.tar 60.0 Kb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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