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Status |
Public on Feb 01, 2009 |
Title |
Expression data from RNAi SNCA treated human neuroblastoma cell line |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The pre-synaptic protein α-synuclein is a key player in the pathogenesis of Parkinson's disease. Together with accumulation and missfolding of α-synuclein protofibrils serve as seed structures for the aggregation of numerous proteins in the cytoplasm of neuronal cells, the so-called Lewy bodies. Furthermore, missense mutations in the SNCA gene and gene multiplications lead to autosomal dominant forms of familiar PD. However, so far the exact biological role of α-synuclein in normal brain is elusive. To gain more insights into the biological function of this protein we monitored whole genome expression changes in dopaminergic neuroblastoma cells (SH-SY5Y) caused by a 90% reduction of α-synuclein by RNA interference. Keywords: whole genome expression changes through the loss of α-synuclein
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Overall design |
comparison of expression profil changes in cells with normal α-synuclein level (control siRNA treatment) and reduced α-synuclein level (siSNCA4 treatment), triplicatesof each condition
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Contributor(s) |
Bonin M, Häbig K |
Citation(s) |
19135032 |
Submission date |
Dec 06, 2007 |
Last update date |
Mar 25, 2019 |
Contact name |
Karina Haebig |
E-mail(s) |
karina.haebig@med.uni-tuebingen.de
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Organization name |
Institute of Human Genetics
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Department |
Medical Genetics
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Lab |
Microarray Facility
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Street address |
Calwerstr. 7
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City |
Tuebingen |
ZIP/Postal code |
72076 |
Country |
Germany |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (6)
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GSM247508 |
Expression data from RNAi SNCA treated human neuroblastoma cell line |
GSM247513 |
Expression data from RNAi SNCA treated human neuroblastoma cell line_si control_Repl2 |
GSM247514 |
Expression data from RNAi SNCA treated human neuroblastoma cell line_transfected with control siRNA_Replicate 3 |
GSM247515 |
Expression data from RNAi SNCA treated human neuroblastoma cell line_transfected with siSNCA4_Replicate 1 |
GSM247516 |
Expression data from RNAi SNCA treated human neuroblastoma cell line_transfected with siSNCA4_Replicate 2 |
GSM247517 |
Expression data from RNAi SNCA treated human neuroblastoma cell line_transfected with siSNCA4_Replicate 3 |
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Relations |
BioProject |
PRJNA103753 |