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| Status |
Public on Oct 11, 2017 |
| Title |
Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The human cerebral cortex possess distinct structural and functional features that are not found in the lower species traditionally used to model brain development and disease. Accordingly, considerable attention has been placed on the development of methods to direct pluripotency stem cells to form human brain-like structures termed organoids. However, many organoid differentiation protocols are inefficient and display marked variability in their ability to recapitulate the three-dimensional architecture and course of neurogenesis in the developing human brain. Here, we report optimized organoid culture methods that efficiently and reliably produce cortical and basal ganglia structures similar to those in the human fetal brain in vivo. Neurons within the organoids are functional and exhibit network-like activities. We further demonstrate the utility of the organoid system for modeling the teratogenic effects of Zika virus on the developing brain and identifying new candidate receptors and therapeutic compounds that can mitigate its desructive actions.
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| Overall design |
Human cerebral organoids were differenitated from H9. For ZIKV analyses, ~ 8W-old cortical organoids with mock or ZIKV infection (~MOI 1.67) after 3 days and 5 days of post infection were collected with 3 biological replicates (6 samples total). RNA samples were sent to the UCLA Clinical Microarray Core (CMC) and RNA integrity was confirmed with the Agilent 2100 bioanalyzer. The cDNA libraries were generated using the KAPA stranded mRNA-Seq kits (KAPA Biosystems) and sequenced by Illumina HiSeq 3000, yielding between 27 and 101 million reads per sample.
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| Contributor(s) |
Watanabe M, Buth JE, Vishlaghi N, de la Torre-Ubeita L, Taxidis J, Khakh B, Coppola G, Pearson CA, Yamauchi K, Gong D, Dai X, Damoiseaux R, Aliyari R, Liebscher S, Schenke-Layland K, Geschwind DH, Cheng G, Golshani P, Sun R, Novitch BG |
| Citation(s) |
29020636 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 NS089817 |
Regulation of neural progenitor functions underlying cortical growth & complexity |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
BENNETT G NOVITCH |
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| Submission date |
Apr 18, 2017 |
| Last update date |
Mar 07, 2023 |
| Contact name |
Bennett Novitch |
| E-mail(s) |
bnovitch@ucla.edu, bnovitch@g.ucla.edu, bnovitch@mednet.ucla.edu
|
| Phone |
3107949339
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| Organization name |
University of California, Los Angeles
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| Department |
Department of Neurobiology
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| Lab |
Novitch Lab
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| Street address |
650 Charles E Young Dr. S, CHS 66-200
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| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE104279 |
Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection |
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| Relations |
| BioProject |
PRJNA383338 |
| SRA |
SRP104155 |
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