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Series GSE97498 Query DataSets for GSE97498
Status Public on Jul 19, 2017
Title Comparison of the RNA expression profiles of CD14+ monocytes from human peripheral blood with derived macrophages (MACs) and osteoclasts (OCs) obtained by exposure with M-CSF and M-CSF/RANKL, respectively, during 5 and 20 days
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The expression profiles of RNA of human CD14+ monocytes were compared with derived macrophages (MACs) and osteoclats (OCs) following M-CSF and M-CSF/RANKL incubation for 5 and 20 days using the SurePrint G3 Human Gene Expression Array (Agilent Technologies, CA, USA). This platform allows the interrogation of >36,000 transcrits and variants per sample. The samples were hybridized in the array following the manufacturer’s instructions.
Overall design Total RNA isolated by standard procedures from CD14+ cells (total monocytes, MOs) corresponding to three sets of samples of monocytes (MOs) and derived MACs and OCs following incubation with M-CSF or M-CSF/RANKL for 5 and 20 days.
Contributor(s) Garcia-Gomez A, Li T, Kerick M, Català-Moll F, Comet NR, Rodríguez-Ubreva J, de la Rica L, Branco MR, Martín J, Ballestar E
Citation(s) 28973458
Submission date Apr 07, 2017
Last update date Nov 27, 2018
Contact name Esteban Ballestar
Organization name Josep Carreras Research Institute (IJC)
Lab Epigenetics and Immune Disease
Street address Ctra de Can Ruti, Camí de les Escoles s/n
City Badalona, Barcelona
State/province N/A = Not Applicable
ZIP/Postal code 08916
Country Spain
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (15)
GSM2570279 CD14_monocyte_donor 1
GSM2570280 CD14_monocyte_donor 2
GSM2570281 CD14_monocyte_donor 3
BioProject PRJNA382125

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97498_RAW.tar 5.0 Mb (http)(custom) TAR
GSE97498_matrix_processed.txt.gz 1.3 Mb (ftp)(http) TXT
GSE97498_matrix_raw.txt.gz 1.8 Mb (ftp)(http) TXT
Processed data are available on Series record

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