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| Status |
Public on Jul 19, 2017 |
| Title |
Comparison of the RNA expression profiles of CD14+ monocytes from human peripheral blood with derived macrophages (MACs) and osteoclasts (OCs) obtained by exposure with M-CSF and M-CSF/RANKL, respectively, during 5 and 20 days |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The expression profiles of RNA of human CD14+ monocytes were compared with derived macrophages (MACs) and osteoclats (OCs) following M-CSF and M-CSF/RANKL incubation for 5 and 20 days using the SurePrint G3 Human Gene Expression Array (Agilent Technologies, CA, USA). This platform allows the interrogation of >36,000 transcrits and variants per sample. The samples were hybridized in the array following the manufacturer’s instructions.
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| Overall design |
Total RNA isolated by standard procedures from CD14+ cells (total monocytes, MOs) corresponding to three sets of samples of monocytes (MOs) and derived MACs and OCs following incubation with M-CSF or M-CSF/RANKL for 5 and 20 days.
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| Contributor(s) |
Garcia-Gomez A, Li T, Kerick M, Català-Moll F, Comet NR, Rodríguez-Ubreva J, de la Rica L, Branco MR, Martín J, Ballestar E |
| Citation(s) |
28973458 |
| Submission date |
Apr 07, 2017 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Esteban Ballestar |
| Organization name |
Josep Carreras Research Institute (IJC)
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| Lab |
Epigenetics and Immune Disease
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| Street address |
Ctra de Can Ruti, Camí de les Escoles s/n
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| City |
Badalona, Barcelona |
| State/province |
N/A = Not Applicable |
| ZIP/Postal code |
08916 |
| Country |
Spain |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA382125 |
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