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| Status |
Public on Dec 14, 2007 |
| Title |
Regulation of gene expression in MDAMB231 breast cancer cells by Parvin-beta in 2D vs 3D culture conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Parvin-beta is a focal adhesion protein downregulated in human breast cancer cells. Loss of Parvin-beta contributes to increased integrin-linked kinase activity, cell-matrix adhesion, and invasion through the extracellular matrix in vitro. The effect of ectopic Parvin-beta expression on the transcriptional profile of MDA-MB-231 breast cancer cells, which normally do not express Parvin-beta was evaluated. Particular emphasis was placed upon propagating MDA-MB-231 breast cancer cells in three-dimensional culture matrices. Gene expression profiles of vector control and Parvin-beta transfected MDA-MB-231 cells cultured on (A) monomeric type I collagen coated plastic, (B) embedded in a type I collagen gel, and (C) embedded in basement membrane (growth factor reduced Matrigel), were compared. Interestingly, Parvin-beta re-expression in MDA-MB-231 cells increased the mRNA expression, serine 82 phosphorylation (mediated by CDK9), and activity of the nuclear hormone receptor, peroxisome proliferator-activated receptor gamma (PPARgamma) and a concomitant increase in lipogenic gene expression as a downstream effector of PPARgamma. Importantly, Parvin-beta suppressed breast cancer growth in vivo with associated decreased proliferation. These data suggest that Parvin-beta might influence breast cancer progression..
Keywords: Gene expression profiling in two dimensional vs three dimensional cell culture
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| Overall design |
The MDA-MB-0231 transfectants were cultured for 7 days in each growth condition and total RNA isolated using Trizol reagent (Invitrogen). Genes either upregulated or downregulated in Parvin-beta transfectants versus vector control cells were compared among the 3 different growth conditions. Venn diagrams were generated using GeneSpring software and used to produce gene lists that were unique to a particular growth condition, or shared between two or all three growth conditions. The focus was primarily on gene expression alterations observed in the 3D collagen type I and 3D Matrigel conditions.
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| Contributor(s) |
Johnstone CN, Mongroo PS, Hannigan GE, Rustgi AK |
| Citation(s) |
17998334 |
| Submission date |
Dec 02, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Cameron N Johnstone |
| E-mail(s) |
cameron.johnstone@petermac.org
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| Organization name |
Peter MacCallum Cancer Centre
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| Department |
Research Division
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| Lab |
Cancer Metastasis Laboratory
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| Street address |
2 St. Andrew's Place
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| City |
East Melbourne |
| State/province |
Vic |
| ZIP/Postal code |
3002 |
| Country |
Australia |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA103669 |
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