GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE96877 Query DataSets for GSE96877
Status Public on Feb 13, 2018
Title Methylation of Transcription Factor YY2 Regulates its Transcriptional Activity and Cell Proliferation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Yin Yang 1 (YY1) is a multifunctional DNA-binding transcription factor shown to be critical in a variety of biological processes, and its activity and function have been shown to be regulated by multitude of mechanisms, which include but are not limited to post-translational modifications (PTMs), its associated proteins and cellular localization. YY2, the paralog of YY1 in mouse and human, has been proposed to function redundantly or oppositely in a context specific manner compared to YY1. Despite its functional importance, how YY2’s DNA binding activity and function is regulated, particularly by PTMs, remains completely unknown. Here we report the first PTM with functional characterization on YY2, namely lysine 247 mono-methylation (K247me1), which was found to be dynamically regulated by SET7/9 and LSD1 both in vitro and in cultured cells. Functional study revealed that SET7/9-mediated YY2 methylation regulated its DNA-binding activity in vitro and association with chromatin examined by ChIP-seq (chromatin immunoprecipitation coupled with sequencing) in cultured cells. Knockout of YY2, SET7/9 or LSD1 by CRISPR (clustered, regularly interspaced, short palindromic repeats) /Cas9-mediated gene editing followed by RNA-seq revealed that a subset of genes was positively-regulated by YY2 and SET7/9, but negatively-regulated by LSD1, which were enriched with genes involved in cell proliferation regulation. Importantly, YY2-regulated gene transcription, cell proliferation and tumor growth were dependent, at least partially, on YY2 K247 methylation. Finally, somatic mutations on YY2 found in cancer, which are in close proximity to K247, altered its methylation, DNA binding activity and gene transcription it controls. Our findings revealed the first PTM with functional implications imposed on YY2 protein, and linked YY2 methylation with its biological functions.
Overall design The RNA-seq in this study was designed to reveal YY2, SET9 and LSD1 regulated genes in HeLa cells.
Contributor(s) Liu W, Zhang W, Ding J
Citation(s) 29098080
Submission date Mar 21, 2017
Last update date May 15, 2019
Contact name Wen Liu
Organization name Xiamen University
Department School of Pharmaceutical Sciences
Street address Xiang'an South Road, Xiang'an District
City Xiamen
State/province Fujian
ZIP/Postal code 361002
Country China
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (4)
GSM2545495 Hela_CTL_RNA-Seq
GSM2545496 Hela_LSD1_KO_RNA-Seq
GSM2545497 Hela_SET9_KO_RNA-Seq
This SubSeries is part of SuperSeries:
GSE96878 Methylation of Transcription Factor YY2 Regulates its Transcriptional Activity and Cell Proliferation
BioProject PRJNA379974
SRA SRP102251

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE96877_RAW.tar 621.1 Mb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap