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Series GSE96841 Query DataSets for GSE96841
Status Public on Nov 02, 2017
Title Smc3 deacetylation by Hos1 facilitates efficient dissolution of sister chromatid cohesion during anaphase
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Sister chromatid cohesion relies on cohesins, a group of proteins that forms a ring-shaped complex embracing sister chromatids. Cohesion is established during S phase and is removed when cohesin Scc1 is cleaved by the protease separase at anaphase onset. During this process, the cohesin subunit Smc3 undergoes a cycle of acetylation: Smc3 acetylation by Eco1 in S phase stabilizes cohesin association with chromosomes, and its deacetylation by Hos1 in anaphase allows re-use of Smc3 in the next cell cycle. Here we find that Smc3 deacetylation by Hos1 has a more immediate effect in early anaphase of budding yeast. Without Hos1, sister chromatid separation and segregation are significantly delayed. Smc3 deacetylation facilitates removal of cohesins from chromosomes, without changing the Scc1 cleavage efficiency, thus promoting dissolution of cohesion. This action is probably due to disengagement of Smc1–3 heads, prompted by de-repression of their ATPase activity. We suggest Scc1 cleavage per se is insufficient for efficient dissolution of cohesion in early anaphase, but subsequent Smc3 deacetylation, which is triggered by Scc1 cleavage, is also required.
Overall design examination of cohesin subunit SMC1 distribution in S.cerevisiae
Contributor(s) Li S, Tanaka T
Citation(s) 29100057
Submission date Mar 21, 2017
Last update date Jun 19, 2019
Contact name Shuyu Li
Phone +447449821740
Organization name University of Dundee
Department School of Life Sciences
Lab TT Lab/GRE
Street address Dow Street
City Dundee
ZIP/Postal code DD12PD
Country United Kingdom
Platforms (1)
GPL21656 Illumina HiSeq 4000 (Saccharomyces cerevisiae)
Samples (6)
GSM2544914 WT_0
GSM2544915 hos1_aid_0
GSM2544916 WT_10
BioProject PRJNA379912
SRA SRP102218

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Supplementary file Size Download File type/resource
GSE96841_RAW.tar 403.3 Mb (http)(custom) TAR (of TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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