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Series GSE96715 Query DataSets for GSE96715
Status Public on Dec 19, 2017
Title Time-dependent regulation of cellular programming of monocytes by NCOR2 [RNASeq_TK]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Whole transcriptome profiling (RNA-Seq) of a time kinetics experiment containing human monocyte-derived cells, which were activated with IL4 either directly at the start of the culture, or at different hours after an initial activation with GMCSF alone. Cells being activated solely with GMCSF were added as controls
Overall design CD14+ monocytes were FACS-sorted from blood of human healthy donors and later activated in vitro with either GMCSF alone for 72 hours to obtain Mo-GMCSF[IL4 (0h)] cells as controls, with the combination of GMCSF and IL4 for 72 hours or 144 hours to obtain Mo-GMCSF[IL4 (0-72h)] or Mo-GMCSF[IL4 (0-144h)] cells, respectively, or with first GMCSF and then with the combination of GMCSF and IL4 for different durations. For the latter, monocytes were first activated with GMCSF for either 12, 24, 48 or 72 hours, and then with GMCSF plus IL4 until a total activation time of 144 hours. This resulted in Mo-GMCSF[IL4 (12-144h)], Mo-GMCSF[IL4 (24-144h)] , Mo-GMCSF[IL4 (48-144h)] and Mo-GMCSF[IL4 (72-144h)] cells, respectively.
Contributor(s) Sander J, Schmidt SV, Cirovic B, McGovern N, Papantonopoulou O, Hardt A, Aschenbrenner A, Kreer C, Quast T, Xu A, Schmidleithner LM, Theis H, Do L, Lauterbach M, Schulte-Schrepping J, Günther P, Xue J, Baßler K, Ulas T, Klee K, Herresthal S, Krebs W, Martin B, Latz E, Händler K, Kraut M, Kolanus W, Beyer M, Falk C, Burgdorf S, Melosh NA, Ginhoux F, Newell E, Schlitzer A, Schultze JL
Citation(s) 29262348
Submission date Mar 16, 2017
Last update date May 15, 2019
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (20)
GSM2538877 Mo_GMCSF_IL4_0h_rep1
GSM2538878 Mo_GMCSF_IL4_0h_rep2
GSM2538880 Mo_GMCSF_IL4_0h_rep3
This SubSeries is part of SuperSeries:
GSE96719 Time-dependent regulation of cellular programming of monocytes by NCOR2
BioProject PRJNA379453
SRA SRP102019

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Supplementary file Size Download File type/resource
GSE96715_GEO_Schultze_RNASeq_TK_processed_NORM_FILT.txt.gz 937.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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