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Series GSE9638 Query DataSets for GSE9638
Status Public on Nov 24, 2007
Title Gene expression profiles of head and neck squamous cell carcinoma (HNSCC)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Distant metastasis is a major factor associated with poor prognosis in head and neck squamous cell carcinoma (HNSCC), but little is known of its molecular mechanisms. New markers that predict clinical outcome, in particular the ability of primary tumors to develop metastatic tumors, are urgently needed. Here, we identified neurotensin, highly expressed in HNSCCs in comparison with normal tissues, as an invasion-promoting factor in HNSCC by using microarray analysis of clinical samples. Indeed, neurotensin overexpression associated with lymph node metastasis of neck, and tumors exhibiting neurotensin overexpression had a poor distant metastasis-free survival rate. In HNSCC cells which expressed neurotensin receptor 1 (NTSR1), neurotensin promoted cellular invasion, migration, and induction of matrix metalloproteinases transcripts. Disruption of NTSR1 signaling by silencing RNA caused the reversion of the invasion of HNSCC cells. By further microarray analysis using neurotensin-treated cells, neurotensin caused increased expression of genes implicated in tumor progression and metastasis including cell growth, migration, invasion, adhesion, angiogenesis, and apoptosis. Our findings have revealed a critical role of neurotensin/NTSR1 for invasion and migration in the metastatic process of HNSCC. This study raises the possibility that neurotensin/NTSR1 could be used as a possible predictive marker and a molecular therapeutic target in the antimetastatic strategies of HNSCC.
Keywords: Identification of a novel therapeutic target for head and neck squamous cell carcinomas
Overall design A total of 21 primary HNSCC samples were obtained from patients who underwent tumor resection at Chiba University Hospital (Chiba, Japan). 21 samples of normal-appearing mucosa at the margin of the surgical resection several centimeters from the tumor were also collected and designated histologically normal mucosa. 21 HNSCCs and paired normal tissues were used for microarray experiment. The clinicopathological grade was classified according to the criteria of the Japan Society for Head and Neck Cancer. The Ethics Committee of Chiba University approved our study, and informed consent was obtained from all patients for use of their tissue samples and clinical data. Tissue samples were immediately snap frozen in liquid nitrogen and stored at -80°C until use.
Contributor(s) Shimizu S
Citation(s) 18661521, 29423074
Submission date Nov 16, 2007
Last update date Jun 28, 2019
Contact name Satoya Shimizu
Organization name Chiba university
Street address 1-8-1 Inohana, Chuo-ku
City Chiba
ZIP/Postal code 260-8670
Country Japan
Platforms (1)
GPL1291 Hitachisoft AceGene Human Oligo Chip 30K 1 Chip Version
Samples (23)
GSM243500 head and neck squamous cell carcinoma (HNSCC) sampleNo.21
GSM243501 head and neck squamous cell carcinoma (HNSCC) sampleNo.20
GSM243502 head and neck squamous cell carcinoma (HNSCC) sampleNo.19
BioProject PRJNA103497

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Supplementary file Size Download File type/resource
GSE9638_RAW.tar 121.8 Mb (http)(custom) TAR (of TXT)
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