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Series GSE9577 Query DataSets for GSE9577
Status Public on Aug 18, 2008
Title Global proteomic characterization of photosystem II complexes from Synechocystis 6803
Organism Synechocystis sp. PCC 6803
Experiment type Other
Summary In this study, we undertook a global proteomics analysis of isolated PSII complexes, comparing protein profiles of HT3 (C-terminal hexahistidine tagged CP47 protein) to those of deltaPsbV HT3, deltaPsbP HT3, and deltaPsbQ HT3. The sensitivity of these techniques allowed for identification of not only stoichiometric components of active PSII complexes, but also for the identification of proteins that are transiently associated with PSII throughout its lifecycle, such as assembly, repair, or degradation partners. From the results, we identified an operon of unknown function which contains binding domains for photosynthetic cofactors and which we have named the cofactor integration operon (cio). Upon deletion of the operon, photosynthetic capacity is decreased, indicating a function in PSII-mediated activity.
Keywords: proteomic, photosystem II, cyanobacteria
 
Overall design Comparison of wild type (HT3) to 3 mutants (deltaPsbV HT3, delataPsbP HT3, and deltaPsbQ HT3)
 
Citation(s) 18693241, 20858728
Submission date Nov 09, 2007
Last update date Mar 17, 2012
Contact name Eric Agnew Welsh
E-mail(s) ewelsh@biology.wustl.edu
Fax 314-935-6803
Organization name Washington University in Saint Louis
Department Biology
Lab Pakrasi
Street address One Brookings Drive; Campus Box 1137
City Saint Louis
State/province MO
ZIP/Postal code 63130
Country USA
 
Platforms (1)
GPL6115 Global proteomic characterization of photosystem II complexes from Synechocystis sp. PCC 6803
Samples (3)
GSM242040 HT3 delta PsbP
GSM242041 HT3 delta PsbQ
GSM242042 HT3 delta PsbV
Relations
BioProject PRJNA103405

Mutant_Ratios_to_Wild_Type header descriptions
ID Protein name
HT3 delta PsbP # unique peptides
HT3 delta PsbP Mutant / WT ratio
HT3 delta PsbP Log2(ratio)
HT3 delta PsbP SE Log2 ratio
HT3 delta PsbQ # unique peptides
HT3 delta PsbQ Mutant / WT ratio
HT3 delta PsbQ Log2(ratio)
HT3 delta PsbQ SE Log2 ratio
HT3 delta PsbV # unique peptides
HT3 delta PsbV Mutant / WT ratio
HT3 delta PsbV Log2(ratio)
HT3 delta PsbV SE Log2 ratio

Data table
ID HT3 delta PsbP # unique peptides HT3 delta PsbP Mutant / WT ratio HT3 delta PsbP Log2(ratio) HT3 delta PsbP SE Log2 ratio HT3 delta PsbQ # unique peptides HT3 delta PsbQ Mutant / WT ratio HT3 delta PsbQ Log2(ratio) HT3 delta PsbQ SE Log2 ratio HT3 delta PsbV # unique peptides HT3 delta PsbV Mutant / WT ratio HT3 delta PsbV Log2(ratio) HT3 delta PsbV SE Log2 ratio
sll0258 8 0.82 -0.28 0.13 8 0.72 -0.47 0.21
sll0427 10 1.12 0.17 0.12 10 1.08 0.11 0.15 10 0.61 -0.71 0.13
sll0851 11 1 0 0.12 11 1.11 0.15 0.08 11 0.91 -0.13 0.07
sll1194 7 0.92 -0.13 0.2 6 1.12 0.16 0.26
sll1398 1 1.76 0.81 0.37 1 6.65 2.73 0.45 1 0.84 -0.25 0.6
sll1414 2 1.81 0.85 0.16 2 5.62 2.49 0.11 2 2.48 1.31 0.13
sll1638 4 0.68 -0.55 0.17 5 0.35 -1.5 0.17
slr0906 11 1.31 0.39 0.1 12 1.42 0.51 0.11 13 1.26 0.33 0.12
slr0927 6 1.59 0.67 0.21 6 1.63 0.7 0.19 6 1.21 0.28 0.12
slr1311 6 1.28 0.36 0.12 6 1.48 0.57 0.12 6 1.01 0.01 0.11
slr1645 2 1.08 0.11 0.5 2 2.58 1.37 0.19 3 2.17 1.12 0.11
smr0006 1 1.07 0.1 0.14 1 0.84 -0.25 0.09 1 1.19 0.25 0.2
smr0007 1 0.82 -0.28 0.57 1 0.88 -0.18 0.16 1 1.06 0.08 0.18
ssl2598 1 1.18 0.24 0.02 1 1.18 0.24 0.12
ssr3451 3 1.66 0.73 0.42 3 1.81 0.86 0.22 3 0.97 -0.04 0.36
sll0018 2 8.26 3.05 0.12 2 12.69 3.67 0.2 2 6.74 2.75 0.18
sll0020 1 1.77 0.83 0.26 2 2.07 1.05 0.27 2 2.18 1.13 0.36
sll0041 1 1.91 0.93 0.57
sll0080 2 1.7 0.77 0.41 2 1.69 0.76 0.55 2 1.11 0.15 0.4
sll0108 1 0.52 -0.94 0.13 1 0.72 -0.48 0.15 1 0.81 -0.3 0.29

Total number of rows: 218

Table truncated, full table size 11 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9577_Peptide_Identifications_From_MSMS.txt 223.2 Kb (ftp)(http) TXT
GSE9577_Raw_Abundance_Data.txt 130.8 Kb (ftp)(http) TXT
GSE9577_Spectra.zip 162.4 Mb (ftp)(http) ZIP
Processed data included within Sample table
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