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| Status |
Public on Feb 18, 2020 |
| Title |
Developmental Genome-Wide DNA Methylation Asymmetry Between Mouse Placenta and Embryo |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
In early embryos, DNA methylation is remodelled to initiate the developmental program. For mostly unknown reasons, methylation marks are acquired unequally between embryonic and placental cells. To better understand this, we generated high-resolution maps of DNA methylation in mouse mid-gestation (E10.5) embryo and placenta. We uncovered specific subtypes of differentially methylated regions (DMRs) that contribute directly to the developmental asymmetry existing between mid-gestation embryo and placenta. We show that the asymmetry between embryonic and placental DNA methylation patterns occurs rapidly during the acquisition of marks in the post-implanted conceptus (E3.5-E6.5), and that these patterns are long-lasting across subtypes of DMRs throughout prenatal development and in somatic tissues. We reveal that at the peri-implantation stages, the de novo methyltransferase activity of DNMT3B is the main driver of methylation marks on asymmetric DMRs, and that DNMT3B can largely compensate for lack of DNMT3A in the epiblast and extraembryonic ectoderm, whereas DNMT3A can only partially palliate for the absence of DNMT3B. However, as development progresses and as DNMT3A becomes the principal de novo methyltransferase, the compensatory DNA methylation mechanism on DMRs becomes less effective.
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| Overall design |
RRBS analyses of whole E10.5 Embryo and whole E10.5 Placenta. Included are also unpublished RRBS data on two independent Trophoblast Stem (TS) celll lines before vs after differentiation.
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| Contributor(s) |
McGraw S |
| Citation(s) |
32056496 |
| |
| Submission date |
Mar 02, 2017 |
| Last update date |
Feb 18, 2020 |
| Contact name |
Serge McGraw |
| E-mail(s) |
Serge.mcgraw@umontreal.ca
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| Organization name |
CHU Ste-Justine Research Center/Université de Montréal
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| Lab |
McGraw Lab
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| Street address |
3175 Côte Sainte-Catherine, office 5.17.005
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| City |
Montreal |
| State/province |
Qc |
| ZIP/Postal code |
H3T 1C5 |
| Country |
Canada |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA377642 |
| SRA |
SRP100983 |
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