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Series GSE95487 Query DataSets for GSE95487
Status Public on Aug 14, 2017
Title Gene expression profiling of CD45+CD3-CD11b+MHCII+ subsets in P5 (day 5 after birth) and 1.5M (1.5 months after birth) mouse epidermal cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The primary Langerhans cells (LCs) network in the mouse skin epidermis is established within a week after birth by differentiation of skin-seeded embryonic LC precursors into mature LCs. Through binding to distinct receptors, two members of the TGFβ superfamily, BMP7 and TGFβ1, initiate cellular signaling essential for inducing LCs differentiation and for preserving LCs in a quiescent state, respectively. However, how these two processes are regulated remains elusive. Here we show that loss of Cbfβ2, one of two RNA splice variants of the Cbfb gene, results in long-term persistence of embryonic-derived immature LCs after their developmental arrest at the transition into the EpCAM+ stage. This phenotype is caused by selective loss of TGFβ receptor signaling, essential for LCs differentiation with intact signaling, which maintains the quiescent state. Interestingly, skin residential Cbfβ2-defcient LC precursors could differentiate further upon transgenic Cbfβ2 expression, but only around postnatal period and not when it was expressed in one month old mice. Loss of developmental potency in the precursor cells was accompanied by diminished BMP7-BMPR1A signaling. Collectively, our results reveal an essential and selective requirement for the Cbfβ2 variant in LC differentiation, and provide a novel insight into how establishment and homeostasis of the LCs network is regulated.
Overall design Examination of wild type and Cbfβ2 knock out at P5 (day 5 after birth) and 1.5M (1.5 months after birth).
Contributor(s) Tenno M, Taniuchi I, Shiroguchi K
Citation(s) 28814567
Submission date Feb 28, 2017
Last update date May 15, 2019
Contact name Katsuyuki Shiroguchi
Organization name RIKEN
Department Center for Biosystems Dymanics Research
Lab Lab for Prediction of Cell Systems Dynamics
Street address 6-2-3 Furuedai
City Suita
State/province Osaka
ZIP/Postal code 565-0874
Country Japan
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (12)
GSM2515127 P5_wt_1
GSM2515128 P5_wt_2
GSM2515129 P5_wt_3
BioProject PRJNA377256
SRA SRP100841

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Supplementary file Size Download File type/resource
GSE95487_GeneExpression_P5-1.5M_wtKO.txt.gz 279.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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