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Series GSE95383 Query DataSets for GSE95383
Status Public on Jun 13, 2017
Title Expression regulation by Pcgf1 in mouse embryonic stem cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The Polycomb repressive complex 1 (PRC1) is essential for fate decisions of embryonic stem (ES) cells. Emerging evidence suggests that six major variants of PRC1 complex, defined by the mutually exclusive presence of Pcgf subunit, regulate distinct biological processes, yet very little is known about the mechanism by which each version of PRC1 instructs and maintains cell fate. Here, we disrupted the Pcgf1, also known as Nspc1 and one of six Pcgf paralogs, in mouse ES cells by the CRISPR/Cas9 technology. We showed that although these mutant cells were viable and retained normal self-renewal, they displayed severe defects in differentiation in vitro. To gain a better understanding of the role of Pcgf1 in transcriptional control of differentiation, we analysed mRNA profiles from Pcgf1 deficient cells using RNA-seq. In contrast to the canonical role of PRC1 in gene repression, we found that Pcgf1 mainly functioned as a transcriptional activator to drive expression of many genes involved in ectoderm and mesoderm differentiation. Chromatin immunoprecipitation experiments demonstrated that Pcgf1 deletion caused a decrease in Ring1B and its associated H2AK119ub1 mark binding to target genes. Altogether, our results revealed an unexpected function of Pcgf1 in gene activation during ES cell maintenance.
Overall design Pcgf1 KO, wildtype and Pcgf1KO+ Pcgf1_flag mESCs
Contributor(s) Yan Y, Zhao W, Qin J
Citation(s) 28393894
BioProject PRJNA374775
Submission date Feb 26, 2017
Last update date May 15, 2019
Contact name Wukui Zhao
Phone 86-02558641524
Organization name Nanjing University
Department Model Animal Research Center
Lab MOE Key Laboratory of Model Animal for Disease Study
Street address 12 Xuefu Road
City Nanjing
State/province Jiangsu
ZIP/Postal code 210061
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (3)
GSM2509596 Pcgf1 KO RNAseq
GSM2509597 wildtype RNAseq
GSM2509598 Pcgf1KO+ Pcgf1_flag RNAseq
SRA SRP099821

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE95383_RAW.tar 7.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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