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Series GSE9517 Query DataSets for GSE9517
Status Public on Dec 13, 2007
Title Cysteine deprivation in liver cell line
Organism Homo sapiens
Experiment type Expression profiling by array
Summary First experiment: Cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM methionine + 0.1 mM cysteine (complete) or supplemented only with 0.1 mM methionine (cysteine-free). Cells were cultured in either medium for 42 h (Long + Cys; Long -Cys) or in cysteine-free medium for 36 h followed by 6 h in complete medium (Short +Cys)
Second experiment: C3A/HepG2 cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM Met and 0.1 mM Cys (complete) or supplemented only with 0.1 mM Met (cysteine-devoid). Cells were cultured in complete medium for 42 h (Long +Cys) or in complete medium for 36 h followed by cysteine-devoid medium for 6 h (Short -Cys).
Keywords: amino acid deprivation
 
Overall design First experiment: Three plates of cells were cultured under each condition. Cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM methionine + 0.1 mM cysteine (complete) or supplemented only with 0.1 mM methionine (cysteine-free). Cells were cultured in either medium for 42 h (Long + Cys; Long -Cys) or in cysteine-free medium for 36 h followed by 6 h in complete medium (Short +Cys).
Second experiment: C3A/HepG2 cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM Met and 0.1 mM Cys (complete) or supplemented only with 0.1 mM Met (cysteine-devoid). Cells were cultured in complete medium for 42 h (Long +Cys) or in complete medium for 36 h followed by cysteine-devoid medium for 6 h (Short -Cys).
 
Contributor(s) Lee J, Wang W, Dominy JE, Hirschberger LL, Stipanuk MH
Citation(s) 18285520
Submission date Nov 05, 2007
Last update date Mar 25, 2019
Contact name Wei Wang
Organization name Cornell University
Lab Microarray Core
Street address T2-003 Vet Res Tower
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (15)
GSM241045 HepG2/C3A cells cysteine depletion for 42 h (long -Cys) #1
GSM241046 HepG2/C3A cells cysteine depletion for 42 h (long -Cys) #2
GSM241047 HepG2/C3A cells cysteine depletion for 42 h (long -Cys) #3
Relations
BioProject PRJNA103319

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9517_RAW.tar 82.0 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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