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Status |
Public on Dec 13, 2007 |
Title |
Cysteine deprivation in liver cell line |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
First experiment: Cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM methionine + 0.1 mM cysteine (complete) or supplemented only with 0.1 mM methionine (cysteine-free). Cells were cultured in either medium for 42 h (Long + Cys; Long -Cys) or in cysteine-free medium for 36 h followed by 6 h in complete medium (Short +Cys) Second experiment: C3A/HepG2 cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM Met and 0.1 mM Cys (complete) or supplemented only with 0.1 mM Met (cysteine-devoid). Cells were cultured in complete medium for 42 h (Long +Cys) or in complete medium for 36 h followed by cysteine-devoid medium for 6 h (Short -Cys). Keywords: amino acid deprivation
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Overall design |
First experiment: Three plates of cells were cultured under each condition. Cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM methionine + 0.1 mM cysteine (complete) or supplemented only with 0.1 mM methionine (cysteine-free). Cells were cultured in either medium for 42 h (Long + Cys; Long -Cys) or in cysteine-free medium for 36 h followed by 6 h in complete medium (Short +Cys). Second experiment: C3A/HepG2 cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM Met and 0.1 mM Cys (complete) or supplemented only with 0.1 mM Met (cysteine-devoid). Cells were cultured in complete medium for 42 h (Long +Cys) or in complete medium for 36 h followed by cysteine-devoid medium for 6 h (Short -Cys).
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Contributor(s) |
Lee J, Wang W, Dominy JE, Hirschberger LL, Stipanuk MH |
Citation(s) |
18285520 |
Submission date |
Nov 05, 2007 |
Last update date |
Mar 25, 2019 |
Contact name |
Wei Wang |
Organization name |
Cornell University
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Lab |
Microarray Core
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Street address |
T2-003 Vet Res Tower
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City |
Ithaca |
State/province |
NY |
ZIP/Postal code |
14853 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (15)
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GSM241045 |
HepG2/C3A cells cysteine depletion for 42 h (long -Cys) #1 |
GSM241046 |
HepG2/C3A cells cysteine depletion for 42 h (long -Cys) #2 |
GSM241047 |
HepG2/C3A cells cysteine depletion for 42 h (long -Cys) #3 |
GSM241048 |
HepG2/C3A cells cys depletion followed by 6 h +Cys (Short +Cys) #4 |
GSM241049 |
HepG2/C3A cells cys depletion followed by 6 h +Cys (Short +Cys) #5 |
GSM241050 |
HepG2/C3A cells cys depletion followed by 6 h +Cys (Short +Cys) #6 |
GSM241051 |
HepG2/C3A cells control medium for 42 h (Long +Cys) #7 |
GSM241052 |
HepG2/C3A cells control medium for 42 h (Long +Cys)#8 |
GSM241053 |
HepG2/C3A cells control medium for 42 h (Long +Cys)#9 |
GSM241154 |
HepG2/C3A cells complete medium followed by 6 h of cysteine deprivation (Short -Cys) #1 |
GSM241155 |
HepG2/C3A cells complete medium followed by 6 h of cysteine deprivation (Short -Cys) #2 |
GSM241156 |
HepG2/C3A cells complete medium followed by 6 h of cysteine deprivation (Short -Cys) #3 |
GSM241157 |
HepG2/C3A cells control medium for 42 h (Long +Cys) #4 |
GSM241158 |
HepG2/C3A cells control medium for 42 h (Long +Cys) #5 |
GSM241159 |
HepG2/C3A cells control medium for 42 h (Long +Cys) #6 |
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Relations |
BioProject |
PRJNA103319 |