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| Status |
Public on Mar 09, 2018 |
| Title |
NOTCH Signaling Specifies a Transient Arterial-Type Hemogenic Endothelium that Gives Rise to Definitive-Type Hematopoiesis from Human Pluripotent Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Recently, we identified and characterized specific endothelial progenitors with varying hemogenic potential during human pluripotent stem cell differentiation. Based on these studies we established a platform on which we can manipulate NOTCH signaling on these subsets to elucidate the specific role of this signaling pathway during hemogenic endothelial specification, endothelial-to-hematopoietic transition, and definitive hematopoietic specification.
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| |
|
| Overall design |
Manipulate NOTCH signaling during hemogenic endothelial specification, endothelial-to-hematopoietic transition, and definitive hematopoietic specification and expansion.
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| |
|
| Contributor(s) |
Slukvin I, Uenishi G, Swanson S, Moskvin O |
| Citation(s) |
29739946 |
| Submission date |
Feb 17, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Oleg Moskvin |
| Organization name |
University of Wisconsin-Madison
|
| Street address |
1220 Capitol Court
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53715 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (4)
|
| GSM2494882 |
D4+1(DLL1 HE:DLL4-)+5(OP9) lin-CD34+CD43+CD45+ |
| GSM2494883 |
D4+1(DLL1 HE:DLL4-)+5(OP9DLL4) lin-CD34+CD43+CD45+ |
| GSM2494884 |
D4+1(DLL1 HE:DLL4+)+5(OP9DLL4) lin-CD34+CD43+CD45+ |
|
| Relations |
| BioProject |
PRJNA375763 |
| SRA |
SRP100165 |
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