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Series GSE94950 Query DataSets for GSE94950
Status Public on May 02, 2017
Title Poly(A)-ClickSeq resolves CF25-mediated alternative poly-adenylation, HeLa
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Poly(A)-ClickSeq is a new methodology for the sequencing of the 3'UTR/poly(A) tail junction of RNA. We analysed both wild-type and CF25Im knock-down HeLa cells in culture using Poly(A)-ClickSeq to find the distribution of poly(A) sites in these samples and determine the role of CF25Im in poly(A) site selection (alternative poly-adenylation, APA).
 
Overall design RNA was extracted from HeLa cells with or without CF25Im Knock-down, each in triplicates, and sequenced using Poly(A)-ClickSeq and sequencing once on a HiSeq and once on a MiSeq
 
Contributor(s) Routh A, Wagner E, Ji P, Xia Z
Citation(s) 28449108
Submission date Feb 15, 2017
Last update date May 15, 2019
Contact name Zheng Xia
E-mail(s) xiaz@ohsu.edu
Organization name Oregon Health & Science University
Department Department of Biomedical Engineering
Lab Xia Lab
Street address 3181 SW Sam Jackson Park Rd
City Portland
State/province Oregon
ZIP/Postal code 97239
Country USA
 
Platforms (2)
GPL15520 Illumina MiSeq (Homo sapiens)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (11)
GSM2492336 C1 HeLa cells mock
GSM2492337 C2 HeLa cells mock
GSM2492338 C3 HeLa cells mock
Relations
BioProject PRJNA374982
SRA SRP100048

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94950_RAW.tar 3.0 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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