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Series GSE94877 Query DataSets for GSE94877
Status Public on Jan 26, 2022
Title Early and extensive venous arterialization during mammalian embryogenesis
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The cellular evolutions and molecular programs underlying the arteriovenous fate settling of embryonic vascular endothelial cells (ECs) are critical for understanding arteriogenesis and inspiring new approaches for regenerative biology. Using different strategies of single-cell RNA sequencing, we constructed the transcriptional landscape of early arteriovenous EC development in both mouse and human embryos, demonstrating the evolutionary conservation of principal vascular EC types and providing a series of conserved arteriovenous genes. We showed an unexpected diversity of arteriovenous characteristics in morphologically alike vascular plexus and further uncovered two transcriptomically distinct arterial EC types, whereas most of heterologous ligand-receptor pairs were shared by different arterial vasculatures. By computational predicting and further genetic lineage tracing, we revealed the widespread venous arterialization in the mid-gestational mouse embryo proper. Interestingly, we demonstrated at transcriptomic level that Notch1 was dispensable for venous arterialization but required subsequently for the arterial feature strengthening in the arterial plexus ECs. Altogether, our findings unprecedentedly detail the comprehensive single-cell mapping of early embryonic vascular ECs in vivo, decipher an asymmetric arteriovenous characteristics different than that in adults, and reveal an extensive venous-to-arterial fate conversion in the vascular plexus.
 
Overall design Here, we performed both well-based single-cell RNA-sequencing (scRNA-seq) of 2,213 cells in mouse embryos and 966 cells in human embryos and droplet-based scRNA-seq of 10,465 cells in mouse embryos to firstly construct a molecular landscape of embryonic vascular endothelium at single cell resolution. Moreover, we also performed well-based single-cell RNA-sequencing of 144 cells from mouse Dll4-tdTomato reporter model, 240 cells from mouse Unc5b-tdTomato reporter model, 192 cells from one mouse Nr2f2-related lineage tracing model and 112 cells from another mouse Nr2f2-related lineage tracing model, to validate our findings. Finally, we performed single-cell RNA-sequencing of 339 cells by well-based method and 25,147 cells by 10x method from mouse Notch1-knockout model, to decode the role of Notch signaling in arterialization.
 
Contributor(s) Hou S, Li Z, Dong J, Gao Y, Chang Z, Ding X, Liu B, Tang F, Lan Y
Citation(s) 35079138
Submission date Feb 14, 2017
Last update date Jan 27, 2022
Contact name Ji Dong
E-mail(s) dongji5211314@126.com
Organization name Peking University
Street address Yiheyuan 5#
City Beijing
ZIP/Postal code 100871
Country China
 
Platforms (4)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL20795 HiSeq X Ten (Homo sapiens)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (77)
GSM2487706 EC4
GSM2487707 EC5
GSM2487708 EC6
Relations
BioProject PRJNA374719
SRA SRP099659

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94877_Barcode-RT_primers_information.xlsx 11.8 Kb (ftp)(http) XLSX
GSE94877_RAW.tar 138.4 Mb (http)(custom) TAR (of CSV, TXT, XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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