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Series GSE94696 Query DataSets for GSE94696
Status Public on May 10, 2017
Title POLR3G Dependent PolyA+ and smallRNA Transcriptomes in Human Pluripotent Stem Cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary POLR3G is expressed at high levels in human pluripotent stem cells (hPSCs) and is required for maintenance of stem cell state through mechanisms, which are not known in detail. In order to explore how POLR3G regulates stem cell state, we have carried out deep sequencing analysis of polyA+ and smallRNA transcriptomes present in hPSCs and regulated in POLR3G dependent manner. Our data reveals that POLR3G regulates a specific subset of the hPSC transcriptome, including multiple transcripts types, such as protein coding genes, lincRNAs, miRNAs and snoRNAs. The primary function of POLR3G is in the maintenance rather than repression of transcription. The majority of POLR3G polyA+ transcriptome is regulated during differentiation and the key pluripotency factors bind to the promoters of at least 30 % of the POLR3G regulated transcripts. The POLR3G transcriptome is functionally linked to embryonic development and important tasks in cellular maintenance and function. #!/bin/bash
Overall design Transcriptome profiles of wild type and POLR3G siRNA knockdown hESC samples, in replicate and Chip-seq with antibodies for H3K4me3, POLR3G, and Input, in replicate.
Contributor(s) Lund RJ, Rahkonen N, Malonzo M, Kauko L, Emani MR, Kivinen V, Närvä E, Laiho A, Skottman H, Rasool OH, Nykter M, Lähdesmäki H, Lahesmaa R
Citation(s) 28494942
Submission date Feb 08, 2017
Last update date May 15, 2019
Contact name Riikka Lund
Organization name Turku Centre for Biotechnology, University of Turku
Street address Tykistökatu 6
City Turku
ZIP/Postal code FI-20520
Country Finland
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (18)
GSM2481028 H360p62 SCR
GSM2481029 H360p62 SiRNA
GSM2481030 H360p66 SCR
BioProject PRJNA371846
SRA SRP099086

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94696_RAW.tar 2.0 Gb (http)(custom) TAR (of BED, BIGWIG, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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