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Series GSE94474 Query DataSets for GSE94474
Status Public on Jul 13, 2017
Title Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromatin fibres dynamically change their organisation during cell cycle. In interphase nucleus, chromatin fibres are evenly distributed whereas their spatial occupancy are reorganised to form condensed chromosomes in mitosis. This process called chromosome condensation is necessary for an accomplishment of faithful chromosome segregation. One of the Structural Maintenance of Chromosomes complexes, Condensin, is indispensable for chromosome condensation. It remains, however, unknown how Condensin plays its role in shaping mitotic chromosome. Here we show that chromatin fibres change their interacting partners; short-range contacts in interphase nucleus are converted into long-range interactions to shape condensed chromosomes. This conversion of interactions among chromatin fibres results in the formation of larger domains within mitotic chromosomes. Condensin is solely in charge of the conversion and large domain formation in fission yeast mitosis. Our results show how fission yeast Condensin is involved in shaping mitotic chromosomes.
 
Overall design Condensin ChIP-seq samples in mitosis.
 
Contributor(s) Kakui Y
Citation(s) 28936767, 28825727
Submission date Feb 03, 2017
Last update date Oct 07, 2019
Contact name Yasutaka Kakui
E-mail(s) yasukakui@aoni.waseda.jp
Organization name Waseda Institute for Advanced Study
Street address 1-21-1 Nishiwaseda, Shinjuku-ku
City Tokyo
ZIP/Postal code 169-0051
Country Japan
 
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (2)
GSM2476215 Cnd2 ChIP
GSM2476216 Input for Cnd2 ChIP
This SubSeries is part of SuperSeries:
GSE94478 Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast
Relations
BioProject PRJNA369797
SRA SRP098808

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94474_RAW.tar 30.0 Kb (http)(custom) TAR (of XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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