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| Status |
Public on Sep 24, 2017 |
| Title |
Epigenetic mechanisms underlie the crosstalk between growth factors and a steroid hormone [methylation II] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
Growth factors (GFs) suppression by steroid hormones recurs in embryology and is co-opted in pathology. While studying mammary cell migration, which is stimulated by GFs and antagonized by glucocorticoids (GCs), we found that GCs inhibit positive feedback loops activated by GFs and stimulate the reciprocal negative loops. Although no alterations in DNA methylation accompany the transcriptional events instigated by either stimulus, forced demethylation of distal regions broadened the repertoire of inducible genes. Our data indicate that the crosstalk involve transcription factors like p53 and NF-kB, along with reduced pausing (and traveling) of RNA polymerase II (RNAPII) at the promoters (and bodies) of GF-inducible genes. In addition, while GFs hyper-acetylated chromatin at unmethylated promoters and enhancers of genes involved in motility, GCs hypo-acetylated the corresponding regions. In conclusion, stably unmethylated genomic regions that encode feedback regulatory modules and differentially recruit RNAPII and acetylases/deacetylases underlie suppression of growth factor signaling by glucocorticoids.
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| Overall design |
DNA methylation – treatment of mammry cells with different agents for 1 hour: Dexamethasone (DEX), Doxorubicin (DOX), Phorbol Ester (PMA), DMSO, EGF. cells were treated with EGF for additional timepoint of 4 hours. DMSO treatment was used as the control.
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| |
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| Contributor(s) |
Enuka Y |
| Citation(s) |
29036586 |
| |
| Submission date |
Jan 31, 2017 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Yehoshua Enuka |
| E-mail(s) |
ys.enuka@gmail.com
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| Phone |
+972-54-2345623
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| Organization name |
Weizmann Institute of Science
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| Department |
Biological Regulation
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| Lab |
Yarden
|
| Street address |
Herzl 234
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| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE94063 |
Epigenetic mechanisms underlie the crosstalk between growth factors and a steroid hormone |
|
| Relations |
| BioProject |
PRJNA369419 |
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