|
| Status |
Public on Mar 23, 2017 |
| Title |
A family of double-homeodomain transcription factors promotes zygotic genome activation in placental mammals [ChIP-seq_d2] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In metazoans, the fertilized embryo is transcriptionally mostly silent for a few cell divisions, until release of a first major wave of embryonic transcripts by so-called zygotic genome activation (ZGA). Maternally provided ZGA-triggering factors have been identified in Drosophila melanogaster and Danio rerio but their mammalian homologues are still undefined. Here, we reveal that DUX family transcription factors are key to this process in human and mouse. First, human Dux4 and murine Dux are expressed prior to ZGA in both species. Second, both orthologues bind to and activate the promoters of ZGA genes. Third, Dux knockdown hinders the progression of mouse embryos beyond the 2-cell stage. Fourth, it prevents the cycling of mouse embryonic stem (ES) cells through a 2-cell-like state whereas, conversely, Dux overexpression induces the accumulation of these ZGA genes-expressing ES cells. We conclude that DUX proteins are master regulators of mammalian ZGA.
|
| |
|
| Overall design |
ChIP-sequencing analysis for endogenous Trim28 in mESCs and for H3K9me3 in mESC depleted of Trim28 or control
|
| |
|
| Contributor(s) |
De Iaco A, Planet E |
| Citation(s) |
28459456 |
| |
| Submission date |
Jan 31, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Evarist Planet |
| E-mail(s) |
evarist.planet@epfl.ch
|
| Organization name |
EPFL
|
| Lab |
LVG
|
| Street address |
Route Cantonale
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE94325 |
A family of double-homeodomain transcription factors promotes zygotic genome activation in placental mammals. |
|
| Relations |
| BioProject |
PRJNA369391 |
| SRA |
SRP098642 |
Less...
More...