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| Status |
Public on Jul 10, 2017 |
| Title |
Transcriptomic profiling of hemp bast fibres at different developmental stages |
| Organism |
Cannabis sativa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cellulosic phloem fibres, also known as bast fibres, find a wide array of applications in industry, for example as eco-friendly substitutes of glass fibres in the manufacture of biocomposites. Bast fibres are cells characterized by a noteworthy length and by a tertiary cell wall (gelatinous or G-layer) mainly composed of crystalline cellulose. During their differentiation, bast fibres undergo specific developmental stages: the cells initially elongate rapidly by intrusive growth, then they cease elongation and start to thicken. In the light of their importance in fostering a bio-economy, it is desirable to widen our knowledge on the molecular mechanisms underlying the differentiation and maturation of bast fibres. The goal of the present study is to provide a transcriptomic close-up of the key events accompanying bast fibre development. The stages going from rapid elongation to cell wall thickening are here studied in textile hemp (Cannabis sativa L.), a fibre crop of great economic importance.
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| Overall design |
Analysis of the fibre transcriptome of hemp plants age 6 weeks. Samples were taken at the TOP, MID (with the snap point) and BOT. Bast fibres were separated using EtOH 80%, a morar and a pestle. The number of biological replicates is four with 13 plants in each replicates.
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| Contributor(s) |
Guerriero G, Behr M, Legay S, Mangeot-Peter L, Zorzan S, Ghoniem M, Hausman J |
| Citation(s) |
28694530 |
| BioProject |
PRJNA435671 |
| |
| Submission date |
Jan 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Gea Guerriero |
| E-mail(s) |
gea.guerriero@list.lu
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| Phone |
+3522758885096
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| Organization name |
Luxembourg Institute of Science and Technology (LIST)
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| Department |
Environmental Research and Innovation Department
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| Lab |
Plant Biotechnologies
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| Street address |
5, rue Bommel, Z.A.E. Robert Steichen, L-4940
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| City |
Hautcharage |
| State/province |
Luxembourg |
| ZIP/Postal code |
L-4940 |
| Country |
Luxembourg |
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| Platforms (1) |
| GPL22277 |
Illumina MiSeq (Cannabis sativa) |
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| Samples (12)
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| Relations |
| SRA |
SRP098115 |
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