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Series GSE94047 Query DataSets for GSE94047
Status Public on Jun 01, 2017
Title Differences of miRNA expression associated with ΔNp63β overexpressing OSCC cell line SQUU-B
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Recent reports have revealed that EMT is involved in cancer invasion and progression including oral squamous cell carcinoma (OSCC). Previously, we have also studied on the association of p63 gene, a homolog of the p53 tumor suppressor gene, with EMT in OSCC cells. Furthermore, we also demonstrated that down-regulation of ΔNp63 accompanied with EMT and that re-expression of ΔNp63β by stable cDNA transfection in OSCC cell lines reverted the EMT phenotype. However, the detail mechanisms of ΔNp63β-mediated EMT remain to be unclear. Recent studies have shown that several microRNAs (miRNAs) are also involved in EMT process of human cancer by targeting EMT-related transcription factors. We thus sought to identify responsible miRNA associated with ΔNp63β-mediated EMT using miRNA microarray analyses.
 
Overall design As samples for miRNA microarray, we used two clones established from an OSCC cell line SQUU-B, which lacks ΔNp63 expression and displays EMT phenotypes. One is SQUU-BO cell overexpressing ΔNp63β, and another is SQUU-BC cell transfected with empty vector. In addition, SQUU-B EL cells with low expression of E-cadherin were also used to identify the miRNA associated with E-cadherin expression strongly.
 
Contributor(s) Hashiguchi Y, Morifuji M, Goto Y
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Submission date Jan 25, 2017
Last update date Jun 03, 2017
Contact name Yuma Hashiguchi
E-mail(s) hasiguchi0330@dent.kyushu-u.ac.jp
Organization name Kyushu University Faculty of Dental Science
Department Division of Maxillofacial Diagnostic and Surgical Sciences
Lab Section of Oral and Maxillofacial Oncology
Street address 3-1-1, Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (3)
GSM2467506 SQUU-B Over
GSM2467507 SQUU-B
GSM2467508 SQUU-B EL
Relations
BioProject PRJNA368645

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94047_RAW.tar 2.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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