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Series GSE94030 Query DataSets for GSE94030
Status Public on Mar 23, 2017
Title Circularized RNA sequencing of wild-type and Mrpp3 knock out mouse heart mitochondria
Organism Mus musculus
Experiment type Other
Summary Here we find that circularization of RNA prior to deep sequencing enables the discovery and characterization of unprocessed mitochondrial RNAs. Using this approach we identify the most stable processing intermediates and the presence of intermediate processing products that are partially degraded and polyadenylated. Analysis of libraries constructed using RNA from mice lacking the nuclease subunit of the mitochondrial RNase P reveals the identities of stalled processing intermediates, their order of cleavage, and confirms the importance of RNase P (MRPP3) in generating mature mitochondrial RNAs. Using RNA circularization prior to library preparation should provide a generally useful approach to studying RNA processing in many different biological systems.
Overall design RNA from purified mouse mitochondria from wild-type and Mrpp3 knock out mouse hearts was circularized and the resulting libraries were sequenced usingan Illumina MiSeq.
Contributor(s) Kuznetsova I, Siira SJ, Filipovska A, Rackham O
Citation(s) 28201688
Submission date Jan 24, 2017
Last update date Jun 25, 2019
Contact name Stefan J Siira
Organization name Harry Perkins Institute of Medical Research
Department Molecular medicine
Lab Mitochondrial medicine and biology
Street address 6 Verdun St, Nedlands
City Perth
State/province Western Australia
ZIP/Postal code 6009
Country Australia
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (8)
GSM2467286 WT standard CircLigase II
GSM2467287 WT 5x CircLigase II
GSM2467288 cRNA WT rep1
BioProject PRJNA363082
SRA SRP097714

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Supplementary file Size Download File type/resource
GSE94030_RAW.tar 4.4 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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