|
| Status |
Public on Apr 07, 2017 |
| Title |
Domestic chickens activate a piRNA defense against avian leukosis virus |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
PIWI-interacting RNAs (piRNAs) protect the germ line by targeting transposable elements (TEs) through base-pair complementarity. We do not know how piRNAs co-evolve with TEs in chickens. Here we reported that all active TEs in the chicken germ line are targeted by piRNAs, and as TEs lose their activity, the corresponding piRNAs erode away. We observed de novo piRNA birth as host responds to a recent retroviral invasion. Avian leukosis virus (ALV) has endogenized prior to chicken domestication, remains infectious, and threatens poultry industry. Domestic fowl produced piRNAs targeting ALV from a genomic locus that was known to render its host ALV resistant. This genomic locus does not produce piRNAs in undomesticated wild chickens. Our findings uncover rapid piRNA evolution reflecting contemporary TE activity, identify a new piRNA acquisition modality by activating a pre-existing genomic locus, and extend piRNA defense roles to include the period when endogenous retroviruses are still infectious.
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| |
|
| Overall design |
Ribosome profiling and RNA sequencing of rooster testes
|
| |
|
| Contributor(s) |
Li XZ, Sun YH |
| Citation(s) |
28384097 |
| |
| Submission date |
Jan 12, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Xin Li |
| E-mail(s) |
xin_li@urmc.rochester.edu
|
| Organization name |
University of Rochester Med Center
|
| Department |
Biochemistry and biophysics
|
| Lab |
Xin Li
|
| Street address |
601 Elmwood Ave.
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16133 |
Illumina HiSeq 2000 (Gallus gallus) |
|
| Samples (2) |
| GSM2454692 |
xzl.gga.WL.testis.wt.adult.rep1+rept2+rept3.RPF |
| GSM2454693 |
xzl.gga.WL.testis.wt.adult.rep1.RNAseq |
|
| Relations |
| BioProject |
PRJNA361113 |
| SRA |
SRP096694 |
